Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self-assemble into chemically stable amphiphilic fi lms, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and effi cient glass functionalization by spontaneous self-assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2-coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fl uorophore, anti-immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self-assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications.

On-the-Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

GRAVAGNUOLO, ALFREDO MARIA;LONGOBARDI, SARA;GIARDINA, PAOLA;
2015

Abstract

Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self-assemble into chemically stable amphiphilic fi lms, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and effi cient glass functionalization by spontaneous self-assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2-coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fl uorophore, anti-immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self-assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/613382
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 26
social impact