The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.

Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery / Avitabile, Concetta; Accardo, Antonella; Ringhieri, Paola; Morelli, Giancarlo; Saviano, Michele; Montagner, Giulia; Fabbri, Enrica; Gallerani, Eleonora; Gambari, Roberto; Romanelli, Alessandra. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 26:8(2015), pp. 1533-41-1541. [10.1021/acs.bioconjchem.5b00156]

Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery

AVITABILE, CONCETTA;ACCARDO, ANTONELLA;RINGHIERI, PAOLA;MORELLI, GIANCARLO;SAVIANO, MICHELE;ROMANELLI, ALESSANDRA
2015

Abstract

The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
2015
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery / Avitabile, Concetta; Accardo, Antonella; Ringhieri, Paola; Morelli, Giancarlo; Saviano, Michele; Montagner, Giulia; Fabbri, Enrica; Gallerani, Eleonora; Gambari, Roberto; Romanelli, Alessandra. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 26:8(2015), pp. 1533-41-1541. [10.1021/acs.bioconjchem.5b00156]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/613304
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