The Biohydrogen Potential (BHP) of six different types of waste biomass typical for the Campania Region (Italy) was investigated. Anaerobic sludge pre-treated with the specific methanogenic inhibitor sodium 2-bromoethanesulfonic acid (BESA) was used as seed inoculum. The BESA pre-treatment yielded the highest BHP in BHP tests carried out with pre-treated anaerobic sludge using potato and pumpkin waste as the substrates, in comparison with aeration or heat shock pre-treatment. The BHP tests carried out with different complex waste biomass showed average BHP values in a decreasing order from potato and pumpkin wastes (171.1±7.3 ml H2/g VS) to buffalo manure (135.6±4.1 ml H2/g VS), dried blood (slaughter house waste, 87.6±4.1 ml H2/g VS), fennel waste (58.1±29.8 ml H2/g VS), olive pomace (54.9±5.4ml H2/g VS) and olive mill wastewater (46.0±15.6ml H2/g VS). The digestate was analyzed for major soluble metabolites to elucidate the different biochemical pathways in the BHP tests. These showed the H2 was produced via mixed type fermentation pathways.

Dark fermentation of complex waste biomass for biohydrogen production by pretreated thermophilic anaerobic digestate

FRUNZO, LUIGI;D'ANTONIO, GIUSEPPE;Esposito G.;PIROZZI, FRANCESCO
2015

Abstract

The Biohydrogen Potential (BHP) of six different types of waste biomass typical for the Campania Region (Italy) was investigated. Anaerobic sludge pre-treated with the specific methanogenic inhibitor sodium 2-bromoethanesulfonic acid (BESA) was used as seed inoculum. The BESA pre-treatment yielded the highest BHP in BHP tests carried out with pre-treated anaerobic sludge using potato and pumpkin waste as the substrates, in comparison with aeration or heat shock pre-treatment. The BHP tests carried out with different complex waste biomass showed average BHP values in a decreasing order from potato and pumpkin wastes (171.1±7.3 ml H2/g VS) to buffalo manure (135.6±4.1 ml H2/g VS), dried blood (slaughter house waste, 87.6±4.1 ml H2/g VS), fennel waste (58.1±29.8 ml H2/g VS), olive pomace (54.9±5.4ml H2/g VS) and olive mill wastewater (46.0±15.6ml H2/g VS). The digestate was analyzed for major soluble metabolites to elucidate the different biochemical pathways in the BHP tests. These showed the H2 was produced via mixed type fermentation pathways.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/598992
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