One of the main issues in biosensor research concerns the control of both the amount and the orientation of the bioreceptors. The so-called photonic induced immobilization can be very effective in anchoring antibodies with the variable part preferentially exposed, thus increasing the sensitivity of a Quartz Crystal Microbalance (QCM) immunosensor. In the present paper such a technique is applied to the detection of parathion, a relatively light analyte, demonstrating that the QCM is able to provide a detectable signal only if the photonic induced immobilization is applied, being completely insensitive to parathion when the bioreceptors are left to adhere randomly. ?????? 2014 Springer Science+Business Media.
Effective antibody anchoring on gold plate by ultra-short UV pulses / B., Ventura; R., Funari; S., Lettieri; Esposito, Rosario; Altucci, Carlo; Velotta, Raffaele. - 162 LNEE:(2014), pp. 119-123. (Intervento presentato al convegno First National Conference on Sensors nel 2014) [10.1007/978-1-4614-3860-1_20].
Effective antibody anchoring on gold plate by ultra-short UV pulses
S. Lettieri;ESPOSITO, ROSARIO;ALTUCCI, CARLO;VELOTTA, RAFFAELE
2014
Abstract
One of the main issues in biosensor research concerns the control of both the amount and the orientation of the bioreceptors. The so-called photonic induced immobilization can be very effective in anchoring antibodies with the variable part preferentially exposed, thus increasing the sensitivity of a Quartz Crystal Microbalance (QCM) immunosensor. In the present paper such a technique is applied to the detection of parathion, a relatively light analyte, demonstrating that the QCM is able to provide a detectable signal only if the photonic induced immobilization is applied, being completely insensitive to parathion when the bioreceptors are left to adhere randomly. ?????? 2014 Springer Science+Business Media.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
