Objectives: MTA cements are hydraulic calcium silicate cements currently used to preserve the vitality of the pulp tissue in direct pulp capping as able to set in the presence of blood and other biological fluids. The study was aimed to develop innovative biphasic calcium silicate/calcium phosphate (MTA-like) cements with hydraulic biointeractive and bioactive properties, able to induce the formation of calcium phosphate minerals and to exert a positive role on pulp cells providing positive epigenetic chemical signals. So, the chemical-physical properties of two calcium silicate/calcium phosphate cements and their effects on odontoblastic differentiation of human dental pulp cells (HDPCs) were investigated. Methods: Two biphasic calcium silicate/calcium phosphate hydraulic cements (CaSi-alphaTCP and CaSi-DCDP) were designed and prepared. The materials were tested for their ion-releasing ability (Ca, P, OH), solubility, water sorption and porosity and for the ability to form calcium phosphate minerals [1-3]. Primary human pulp cells (HPC) were cultured with the extracts of the cements. The upregulation of alkaline phosphatese (ALP) and osteocalcin (OCN) was examined after 24-h culture by quantitative real-time PCR and results expressed as fold change [4]. Results: The chemical-physical data showed high release of Ca and OH ions (tables a,b), high open pore volume and apparent porosity. ESEM-EDX results demonstrated a pronounced ability to nucleate calcium phosphates on their surface. Pulp cells treated with CaSi-alphaTCP cement showed a strong upregulation of ALP and OCN genes, namely ten-fold increase for OCN and three-fold increase for ALP compared to the control cells. Differently, no variations in gene regulation were induced by CaSi-DCDP cement (graphics c,d). Conclusions: Biphasic calcium silicate/calcium phosphate cements showed high biointeractivity (release of Ca and OH ions) in correlation with their marked ability to nucleate calcium phosphates. Cells data suggest the CaSi-alphaTCP cement is a potent inducer of genes such as ALP and OCN as characteristic markers of the mineralization processes normally little expressed in the HPC. These innovative cements may provide epigenetic signals to promote human dental pulp cells differentiation and to induce the formation of the dentine bridge, and appear attractive materials for pulp-capping applications.

Experimental biphasic Calcium Silicate/Calcium Phosphate cements for direct pulp capping / Gandolfi, Mg; Spagnuolo, Gianrico; Siboni, F.; Procino, A; Riccitiello, Francesco; Prati, C; Rengo, S.. - (2014). (Intervento presentato al convegno XXI Congresso Nazionale Collegio Docenti di Odontoiatria tenutosi a ROMA nel 10 -12 Aprile 2014).

Experimental biphasic Calcium Silicate/Calcium Phosphate cements for direct pulp capping

SPAGNUOLO, GIANRICO;RICCITIELLO, FRANCESCO;RENGO S.
2014

Abstract

Objectives: MTA cements are hydraulic calcium silicate cements currently used to preserve the vitality of the pulp tissue in direct pulp capping as able to set in the presence of blood and other biological fluids. The study was aimed to develop innovative biphasic calcium silicate/calcium phosphate (MTA-like) cements with hydraulic biointeractive and bioactive properties, able to induce the formation of calcium phosphate minerals and to exert a positive role on pulp cells providing positive epigenetic chemical signals. So, the chemical-physical properties of two calcium silicate/calcium phosphate cements and their effects on odontoblastic differentiation of human dental pulp cells (HDPCs) were investigated. Methods: Two biphasic calcium silicate/calcium phosphate hydraulic cements (CaSi-alphaTCP and CaSi-DCDP) were designed and prepared. The materials were tested for their ion-releasing ability (Ca, P, OH), solubility, water sorption and porosity and for the ability to form calcium phosphate minerals [1-3]. Primary human pulp cells (HPC) were cultured with the extracts of the cements. The upregulation of alkaline phosphatese (ALP) and osteocalcin (OCN) was examined after 24-h culture by quantitative real-time PCR and results expressed as fold change [4]. Results: The chemical-physical data showed high release of Ca and OH ions (tables a,b), high open pore volume and apparent porosity. ESEM-EDX results demonstrated a pronounced ability to nucleate calcium phosphates on their surface. Pulp cells treated with CaSi-alphaTCP cement showed a strong upregulation of ALP and OCN genes, namely ten-fold increase for OCN and three-fold increase for ALP compared to the control cells. Differently, no variations in gene regulation were induced by CaSi-DCDP cement (graphics c,d). Conclusions: Biphasic calcium silicate/calcium phosphate cements showed high biointeractivity (release of Ca and OH ions) in correlation with their marked ability to nucleate calcium phosphates. Cells data suggest the CaSi-alphaTCP cement is a potent inducer of genes such as ALP and OCN as characteristic markers of the mineralization processes normally little expressed in the HPC. These innovative cements may provide epigenetic signals to promote human dental pulp cells differentiation and to induce the formation of the dentine bridge, and appear attractive materials for pulp-capping applications.
2014
Experimental biphasic Calcium Silicate/Calcium Phosphate cements for direct pulp capping / Gandolfi, Mg; Spagnuolo, Gianrico; Siboni, F.; Procino, A; Riccitiello, Francesco; Prati, C; Rengo, S.. - (2014). (Intervento presentato al convegno XXI Congresso Nazionale Collegio Docenti di Odontoiatria tenutosi a ROMA nel 10 -12 Aprile 2014).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/595525
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