RESIN REGULATION OF IL6 AND RELATED RECEPTORS TRANSCRIPTION IN HGFs

Objective: The primary aim of the present in vitro study was the assessment of the potential cytotoxicity of self-polymerizing dental resins on human gingival fibroblasts (HGFs) isolated from healthy patients. The secondary goal of this investigation was the evaluation of the quantitative gene expression of IL6, and its receptors (gp80 and gp130). Method: Three different self-polymerizing dental resins (JetKit, Duralay, Unifast III) were analyzed. HGFs were isolated from fragments of gingival tissues removed from healthy patients. The cytotoxic activity analysis on HGFs was made using standard MTT-test (3,[4,5-dimethylthiazol-2]2,5 difeniltetrazolium bromide). Conversely, the quantitative determinations of IL6, gp80, gp130 gene expression were evaluated by means of QRT-PCR on a StepOne System. The quantification was made in 48-well plates with a TaqMan Universal MasterMix. Statistical analysis of data were performed by ANOVA. Result: MTT data showed that all the materials tested caused a not significant toxic effect in HGFs. As to the QRT-PCR, IL6, gp80 and gp130 genes were upregulated in HGFs stimulated with all the tested dental resins. Furthermore, IL6 and its receptors were overexpressed in HGFs treated with JetKit compared to HGFs treated with Duralay and Unifast III. Conclusion: Our results showed that all tested self-polymerizing dental resins materials involved IL6 as a potential regulator of the epigenetic mechanisms by means of the inflammatory process.