A straightforward way to measure separated micrometric sized particles in microfluidic flow is reported. The light scattering profile (LSP) of each single particle is fully characterized by using a CMOS-camera based small angle light scattering (SALS) apparatus, ranging from 2° up to 30°. To ensure controlled particle passage through the incident laser, a viscoelastic 3D alignment effect by viscoelastic induced particle migration has been implemented in a simple and cost-effective microfluidic device. Different polystyrene particle sizes are measured in microfluidic flows and the obtained scattering signatures are matched with the Lorenz-Mie based scattering theory. The results confirm the possibility of using this apparatus for real multiplex particle analyses in microfluidic particle flows
Multiplex single particle analysis in microfluidics / Dannhauser, David; Romeo, G; Causa, Filippo; DE SANTO, Ilaria; Netti, PAOLO ANTONIO. - In: ANALYST. - ISSN 0003-2654. - 139:(2014), pp. 5239-5246. [10.1039/C4AN01033G]
Multiplex single particle analysis in microfluidics
DANNHAUSER, DAVID;CAUSA, FILIPPO;DE SANTO, ILARIA;NETTI, PAOLO ANTONIO
2014
Abstract
A straightforward way to measure separated micrometric sized particles in microfluidic flow is reported. The light scattering profile (LSP) of each single particle is fully characterized by using a CMOS-camera based small angle light scattering (SALS) apparatus, ranging from 2° up to 30°. To ensure controlled particle passage through the incident laser, a viscoelastic 3D alignment effect by viscoelastic induced particle migration has been implemented in a simple and cost-effective microfluidic device. Different polystyrene particle sizes are measured in microfluidic flows and the obtained scattering signatures are matched with the Lorenz-Mie based scattering theory. The results confirm the possibility of using this apparatus for real multiplex particle analyses in microfluidic particle flowsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.