The regulations of some synthesis genes, and those of recycling and oxidation, related to ascorbic acid were studied during ripening and storage in Campanian tomato ecotypes Vesuvio (V) and Tondo Giallo (TG) with long shelf‐life, and perishable Maiese (M). At ripening (R), in V and TG gene expressions were similar or lower respect to M, while total ascorbic acid (tAsA: ascorbic, AsA, and dehydroascorbic, DhA, acids) and AsA were higher, and AsA/tAsA was lower than in M. During ripening tAsA and AsA increased in V and TG, and decreased in M. These metabolite trends disagree with those of gene expressions, therefore, more investigations on alternative synthesis roots and enzyme activities are needed. However, the higher tAsA in V and TG at R agreed with the recorded ACCS (ethylene synthesis key gene) downregulation respect to M, in latter a higher ethylene activity, as known, could have lowered tAsA, and could explain softer fruit observed. Further, ethylene downregulates synthesis genes and no recycling ones, in agreement with evidences in relative gene expressions detected in the ecotypes. During storage, all genes in M and most part of them in V were with similar expression levels and downregulated respect to levels at R. In TG many synthesis genes and oxidation or recycle genes were upregulated respect to levels at R and to those of M. The tAsA content, almost similar among ecotypes, remained as at R in M, fell down and after remained constant in V, and gradually decreased in TG. The AsA/DhA was higher in V and TG than in M. Respect to the level at R, ACCS was downregulated in M, remained low expressed in V, and upregulated in TG. In M a higher ethylene could have triggered oxidative burst at R that could be maintained during storage, inducing AsA oxidation overcame DhA recycle. Instead, the lower, in V, or delayed, in TG, ethylene activity during ripening and storage, and in TG also the good reducing level by ascorbic acid, could be related to the long shelf‐life.
Regulation of ascorbic acid accumulation in tomato ecotypes during ripening and storage / Maria, Minutolo; Anna, Zarrillo; Elena, Gazzillo; Amalfitano, Carmine; Errico, Angela. - (2014), pp. 56-56. (Intervento presentato al convegno XXXII CONVEGNO NAZIONALE Società Italiana di Chimica Agraria tenutosi a Bolzano nel 7-9 settembre 2014).
Regulation of ascorbic acid accumulation in tomato ecotypes during ripening and storage
AMALFITANO, CARMINE;ERRICO, ANGELA
2014
Abstract
The regulations of some synthesis genes, and those of recycling and oxidation, related to ascorbic acid were studied during ripening and storage in Campanian tomato ecotypes Vesuvio (V) and Tondo Giallo (TG) with long shelf‐life, and perishable Maiese (M). At ripening (R), in V and TG gene expressions were similar or lower respect to M, while total ascorbic acid (tAsA: ascorbic, AsA, and dehydroascorbic, DhA, acids) and AsA were higher, and AsA/tAsA was lower than in M. During ripening tAsA and AsA increased in V and TG, and decreased in M. These metabolite trends disagree with those of gene expressions, therefore, more investigations on alternative synthesis roots and enzyme activities are needed. However, the higher tAsA in V and TG at R agreed with the recorded ACCS (ethylene synthesis key gene) downregulation respect to M, in latter a higher ethylene activity, as known, could have lowered tAsA, and could explain softer fruit observed. Further, ethylene downregulates synthesis genes and no recycling ones, in agreement with evidences in relative gene expressions detected in the ecotypes. During storage, all genes in M and most part of them in V were with similar expression levels and downregulated respect to levels at R. In TG many synthesis genes and oxidation or recycle genes were upregulated respect to levels at R and to those of M. The tAsA content, almost similar among ecotypes, remained as at R in M, fell down and after remained constant in V, and gradually decreased in TG. The AsA/DhA was higher in V and TG than in M. Respect to the level at R, ACCS was downregulated in M, remained low expressed in V, and upregulated in TG. In M a higher ethylene could have triggered oxidative burst at R that could be maintained during storage, inducing AsA oxidation overcame DhA recycle. Instead, the lower, in V, or delayed, in TG, ethylene activity during ripening and storage, and in TG also the good reducing level by ascorbic acid, could be related to the long shelf‐life.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
