Silybin A and B were separated from commercial silibinin using the preparative HPLC method. The described method is rapid and effective in obtaining gram-scale amounts of two diastereoisomers with minimal effort. In our approach, silibinin was dissolved in THF (solubility greater than 100 mg/mL), an alternative solvent to H2O or MeOH in which silibinin has a very low solubility (ca 0.05-1.5 mg/mL), and then separated into its two components using preparative RP-HPLC. By starting with purified diastereoisomers, it was possible to obtain the two enantiomers of 2,3-dehydrosilybin in good yields and optically pure using an efficient oxidation procedure. All of the purified products were fully characterised using NMR (1H, 13C), CD, [??]D, and ESI MS analyses. The purities of the products, which were evaluated using analytical HPLC, were greater than 98 % in all cases.

A rapid and simple chromatographic separation of diastereomers of silibinin and their oxidation to produce 2,3-dehydrosilybin enantiomers in an optically pure form

DI FABIO, GIOVANNI;ROMANUCCI, VALERIA;DI MARINO, CINZIA;DE NAPOLI, LORENZO;ZARRELLI, ARMANDO
2013

Abstract

Silybin A and B were separated from commercial silibinin using the preparative HPLC method. The described method is rapid and effective in obtaining gram-scale amounts of two diastereoisomers with minimal effort. In our approach, silibinin was dissolved in THF (solubility greater than 100 mg/mL), an alternative solvent to H2O or MeOH in which silibinin has a very low solubility (ca 0.05-1.5 mg/mL), and then separated into its two components using preparative RP-HPLC. By starting with purified diastereoisomers, it was possible to obtain the two enantiomers of 2,3-dehydrosilybin in good yields and optically pure using an efficient oxidation procedure. All of the purified products were fully characterised using NMR (1H, 13C), CD, [??]D, and ESI MS analyses. The purities of the products, which were evaluated using analytical HPLC, were greater than 98 % in all cases.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/585503
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