Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.
Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability / Terracciano, Monica; Ilaria, Rea; Luca, Stefano; Ivo, Rendina; Oliviero, Giorgia; Nici, Fabrizia; D'Errico, Stefano; Piccialli, Gennaro; Borbone, Nicola. - In: NANOSCALE RESEARCH LETTERS. - ISSN 1556-276X. - 9:1(2014), p. 317. [10.1186/1556-276X-9-317]
Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
TERRACCIANO, MONICA;OLIVIERO, GIORGIA;NICI, FABRIZIA;D'ERRICO, STEFANO;PICCIALLI, GENNARO;BORBONE, NICOLA
2014
Abstract
Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
