We present some additional results obtained with the BETAview system, a digital system for real time /spl beta/-imaging based on semiconductor pixel detectors and on a single photon counting read-out chip. We describe the system assembled with a Si detector, 300-/spl mu/m thick, segmented into 64 /spl times/64 square pixels with 170-/spl mu/m pitch. The solid-state pixel array detector is bump-bonded pixel by pixel to a low threshold, single particle counting electronics, the photon counting chip (PCC) developed by the Medipix1 Collaboration for biomedical applications. Each cell (corresponding to a detector pixel) of the front-end chip has a maximum count rate of 2 MHz, a minimum nominal threshold of 1400 e/sup -/ (corresponding about 5 keV in Si), and contains a 15-bit counter. The detector sensitive area is about 1 cm/sup 2/, but several detectors could be assembled in arrays. The measured background counts were about 3.5/spl times/10/sup -2/ cps/cm/sup 2/, which implies a very high sensitivity of the device. The detection threshold used in this experiment was /spl sim/15 keV. We show the results obtained in the real-time monitoring of two biological dynamic processes: an amino acid uptake by living cells and the thermal denaturation process of oligonucleotides. Specifically, we have followed over time the accumulation of a marked amino acid ([/sup 14/C]L-Leucine) into Octopus vulgaris eggs cells. The results, described in Section III-B, show that after about 20 min from the beginning of the uptake process, the radioactively marked eggs become clearly visible over the background culture radioactive medium. In Sections III-C and III-D, we also describe the successful performance of the /spl beta/-imaging system in genetic studies involving the real time reconstruction of denaturation and kinetics curves for two different /sup 32/P-radiolabeled nucleotides.

REAL-TIME BETA IMAGING WITH SILICON HYBRID PIXEL DETECTORS: KINETIC MEASUREMENTS WITH C-14 AMINO ACIDS AND P-32 NUCLEOTIDES / Mettivier, Giovanni; Montesi, MARIA CRISTINA; Russo, Paolo; Bertolucci, Ennio; Conti, Maurizio; DI COSMO, Anna; A., Maiorino; Paolella, Giovanni; T., Pecorella; R., Scognamiglio. - In: IEEE TRANSACTIONS ON NUCLEAR SCIENCE. - ISSN 0018-9499. - 49:(2002), pp. 2213-2217. [10.1109/TNS.2002.803811]

REAL-TIME BETA IMAGING WITH SILICON HYBRID PIXEL DETECTORS: KINETIC MEASUREMENTS WITH C-14 AMINO ACIDS AND P-32 NUCLEOTIDES

METTIVIER, GIOVANNI;MONTESI, MARIA CRISTINA;RUSSO, PAOLO;BERTOLUCCI, ENNIO;CONTI, MAURIZIO;DI COSMO, ANNA;PAOLELLA, GIOVANNI;
2002

Abstract

We present some additional results obtained with the BETAview system, a digital system for real time /spl beta/-imaging based on semiconductor pixel detectors and on a single photon counting read-out chip. We describe the system assembled with a Si detector, 300-/spl mu/m thick, segmented into 64 /spl times/64 square pixels with 170-/spl mu/m pitch. The solid-state pixel array detector is bump-bonded pixel by pixel to a low threshold, single particle counting electronics, the photon counting chip (PCC) developed by the Medipix1 Collaboration for biomedical applications. Each cell (corresponding to a detector pixel) of the front-end chip has a maximum count rate of 2 MHz, a minimum nominal threshold of 1400 e/sup -/ (corresponding about 5 keV in Si), and contains a 15-bit counter. The detector sensitive area is about 1 cm/sup 2/, but several detectors could be assembled in arrays. The measured background counts were about 3.5/spl times/10/sup -2/ cps/cm/sup 2/, which implies a very high sensitivity of the device. The detection threshold used in this experiment was /spl sim/15 keV. We show the results obtained in the real-time monitoring of two biological dynamic processes: an amino acid uptake by living cells and the thermal denaturation process of oligonucleotides. Specifically, we have followed over time the accumulation of a marked amino acid ([/sup 14/C]L-Leucine) into Octopus vulgaris eggs cells. The results, described in Section III-B, show that after about 20 min from the beginning of the uptake process, the radioactively marked eggs become clearly visible over the background culture radioactive medium. In Sections III-C and III-D, we also describe the successful performance of the /spl beta/-imaging system in genetic studies involving the real time reconstruction of denaturation and kinetics curves for two different /sup 32/P-radiolabeled nucleotides.
2002
REAL-TIME BETA IMAGING WITH SILICON HYBRID PIXEL DETECTORS: KINETIC MEASUREMENTS WITH C-14 AMINO ACIDS AND P-32 NUCLEOTIDES / Mettivier, Giovanni; Montesi, MARIA CRISTINA; Russo, Paolo; Bertolucci, Ennio; Conti, Maurizio; DI COSMO, Anna; A., Maiorino; Paolella, Giovanni; T., Pecorella; R., Scognamiglio. - In: IEEE TRANSACTIONS ON NUCLEAR SCIENCE. - ISSN 0018-9499. - 49:(2002), pp. 2213-2217. [10.1109/TNS.2002.803811]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/581
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