The behaviour of catalytic reactions was studied when an enzyme is immobilized to a membrane interposed between two solutions of identical substrate concentration, kept at different temperatures. Beta-Galactosidase was studied as a model enzyme. In the presence of a temperature gradient the apparent enzyme activity is higher than in comparable isothermal conditions. The activity increase is proportional to the applied transmembrane temperature difference. Two possible explanations are proposed for this behaviour. Various biotechnological applications are suggested.
Immobilized-enzyme activity under isothermal and non-isothermal conditions: Studies on β-galactosidase as a model enzyme / D., Mita; M., Portaccio; P., Russo; S., Stellato; Toscano, Giuseppe; U., Bencivenga; P., Canciglia; A., D'Acunto; N., Pagliuca; S., Rossi; F., Gaeta. - In: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. - ISSN 0885-4513. - STAMPA. - 22:3(1995), pp. 281-294.
Immobilized-enzyme activity under isothermal and non-isothermal conditions: Studies on β-galactosidase as a model enzyme
TOSCANO, GIUSEPPE;
1995
Abstract
The behaviour of catalytic reactions was studied when an enzyme is immobilized to a membrane interposed between two solutions of identical substrate concentration, kept at different temperatures. Beta-Galactosidase was studied as a model enzyme. In the presence of a temperature gradient the apparent enzyme activity is higher than in comparable isothermal conditions. The activity increase is proportional to the applied transmembrane temperature difference. Two possible explanations are proposed for this behaviour. Various biotechnological applications are suggested.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
