A convenient solid-phase synthesis of oligonucleotides conjugated at the 3'-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked 17-mer, designed for antisense experiments, has been prepared in good yields, and its hybridization properties with a complementary DNA fragment evaluated by UV thermal analysis.
A facile solid phase strategy for the synthesis of oligonucleotide – tetraphenylporphyrin conjugates / DE NAPOLI, Lorenzo; S., De Luca; DI FABIO, Giovanni; A., Messere; Montesarchio, Daniela; Morelli, Giancarlo; Piccialli, Gennaro; Tesauro, Diego. - In: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. - ISSN 1434-193X. - 2000:6(2000), pp. 1013-1018. [10.1002/(SICI)1099-0690(200003)2000:6<1013::AID-EJOC1013>3.0.CO;2-3]
A facile solid phase strategy for the synthesis of oligonucleotide – tetraphenylporphyrin conjugates
DE NAPOLI, LORENZO;DI FABIO, GIOVANNI;MONTESARCHIO, DANIELA;MORELLI, GIANCARLO;PICCIALLI, GENNARO;TESAURO, DIEGO
2000
Abstract
A convenient solid-phase synthesis of oligonucleotides conjugated at the 3'-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked 17-mer, designed for antisense experiments, has been prepared in good yields, and its hybridization properties with a complementary DNA fragment evaluated by UV thermal analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
