Caprine herpesvirus type 1 (CpHV-1), like other members of the alpha subfamily of herpesviruses, establishes latent infections in trigeminal ganglion neurons. Our groups previously demonstrated that CpHV-1 induces apoptosis in goat peripheral blood mononuclear cells and in an epithelial bovine cell line, but the ability of CpHV-1 to induce apoptosis in neuronal cells remains unexplored. In this report, the susceptibility of Neuro 2A cells to infection by CpHV-1 was examined. Following infection of cultured cells with CpHV-1, expression of cell death genes was evaluated using real-time PCR and Western blot assays. Analysis of virus-infected cells revealed activation of caspase-8, a marker for the extrinsic pathway of apoptosis, and caspase-9, a marker for the intrinsic pathway of apoptosis at 12 and 24 h post-infection. Significant increase in the levels of cleaved caspase-3 was also observed at the acme of cytopathic effect at 24 h post-infection. In particular, at 3 and 6 h post-infection, several proapototic genes were under-expressed. At 12 h post-infection several proapototic genes such as caspases, TNF, Cd70, and Traf1 were over expressed while Bcl2a1a, Fadd, and TNF genes were underexpressed. In conclusion, the simultaneous activation of caspase-8 and caspase-9 suggests that CpHV-1 can trigger the death-receptor pathway and the mitochondrial pathway separately and in parallel. Our findings are significant because this is the first published study showing the effect of CpHV-1 infection in neuronal cells in terms of gene expression and apoptosis modulation.

Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line / Montagnaro, Serena; Ciarcia, Roberto; Claudio De, Martinis; Carmen, Pacilio; Sasso, Simona; Maria Valeria, Puzio; Morena De, Angelis; Pagnini, Ugo; Silvia, Boffo; Ivanna, Kenez; Iovane, Giuseppe; Antonio, Giordano. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - 114:12(2013), pp. 2809-2822. [10.1002/jcb.24628]

Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line

MONTAGNARO, SERENA;CIARCIA, ROBERTO;SASSO, SIMONA;PAGNINI, UGO;IOVANE, GIUSEPPE;
2013

Abstract

Caprine herpesvirus type 1 (CpHV-1), like other members of the alpha subfamily of herpesviruses, establishes latent infections in trigeminal ganglion neurons. Our groups previously demonstrated that CpHV-1 induces apoptosis in goat peripheral blood mononuclear cells and in an epithelial bovine cell line, but the ability of CpHV-1 to induce apoptosis in neuronal cells remains unexplored. In this report, the susceptibility of Neuro 2A cells to infection by CpHV-1 was examined. Following infection of cultured cells with CpHV-1, expression of cell death genes was evaluated using real-time PCR and Western blot assays. Analysis of virus-infected cells revealed activation of caspase-8, a marker for the extrinsic pathway of apoptosis, and caspase-9, a marker for the intrinsic pathway of apoptosis at 12 and 24 h post-infection. Significant increase in the levels of cleaved caspase-3 was also observed at the acme of cytopathic effect at 24 h post-infection. In particular, at 3 and 6 h post-infection, several proapototic genes were under-expressed. At 12 h post-infection several proapototic genes such as caspases, TNF, Cd70, and Traf1 were over expressed while Bcl2a1a, Fadd, and TNF genes were underexpressed. In conclusion, the simultaneous activation of caspase-8 and caspase-9 suggests that CpHV-1 can trigger the death-receptor pathway and the mitochondrial pathway separately and in parallel. Our findings are significant because this is the first published study showing the effect of CpHV-1 infection in neuronal cells in terms of gene expression and apoptosis modulation.
2013
Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line / Montagnaro, Serena; Ciarcia, Roberto; Claudio De, Martinis; Carmen, Pacilio; Sasso, Simona; Maria Valeria, Puzio; Morena De, Angelis; Pagnini, Ugo; Silvia, Boffo; Ivanna, Kenez; Iovane, Giuseppe; Antonio, Giordano. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - 114:12(2013), pp. 2809-2822. [10.1002/jcb.24628]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/563604
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