Biomimetic CO2 capture includes environmentally friendly solutions based on carbonic anhydrase (CA), an enzyme that increases CO2 absorption rate in conventional acid-gas scrubbing processes. The present contribution reports the characterization of a new recombinant carbonic anhydrase, SspCA, isolated from the thermophile bacterium Sulphurhydrogenibium yellowstonense sp. YO3AOP1.The kinetics of SspCA was characterized in terms of first order CO2 hydration rate according to a procedure based on CO2 absorption tests in a stirred cell apparatus. The first order kinetic constant at 25��C was 9.16��106L/(mols).An appropriate investigation on SspCA stability was carried out to assess its long-term resistance to high temperatures as in all capture processes based on absorption/vacuum-desorption cycles. Its half-life was 53 and 8 days at 40. ��C and 70. ��C, respectively. �� 2013 Elsevier Inc.
Kinetic study of a novel thermo-stable alfa-carbonic anhydrase for biomimetic CO2 capture / M., Russo; Olivieri, Giuseppe; C., Capasso; V. D., Luca; Marzocchella, Antonio; Salatino, Piero; M., Rossi. - In: ENZYME AND MICROBIAL TECHNOLOGY. - ISSN 0141-0229. - 53:(2013), pp. 271-277. [10.1016/j.enzmictec.2013.05.002]
Kinetic study of a novel thermo-stable alfa-carbonic anhydrase for biomimetic CO2 capture
OLIVIERI, GIUSEPPE;MARZOCCHELLA, ANTONIO;SALATINO, PIERO;
2013
Abstract
Biomimetic CO2 capture includes environmentally friendly solutions based on carbonic anhydrase (CA), an enzyme that increases CO2 absorption rate in conventional acid-gas scrubbing processes. The present contribution reports the characterization of a new recombinant carbonic anhydrase, SspCA, isolated from the thermophile bacterium Sulphurhydrogenibium yellowstonense sp. YO3AOP1.The kinetics of SspCA was characterized in terms of first order CO2 hydration rate according to a procedure based on CO2 absorption tests in a stirred cell apparatus. The first order kinetic constant at 25��C was 9.16��106L/(mols).An appropriate investigation on SspCA stability was carried out to assess its long-term resistance to high temperatures as in all capture processes based on absorption/vacuum-desorption cycles. Its half-life was 53 and 8 days at 40. ��C and 70. ��C, respectively. �� 2013 Elsevier Inc.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
