Urocortin (UCN), a 40 amino acid peptide is a Corticotrophin-Releasing Hormone (CRH) related peptide. The biological actions of CRH family peptides are mediated via two types of G-protein coupled receptors, CRH type 1 (CRHR1) and CRH type 2 (CRHR2). The aim of the present study was to investigate the expression of UCN, CRHR1 and CRHR2 by immunoprecipitation, Western blot, immunohistochemistry and RT-PCR in the bovine thyroid gland. Immunoprecipitation and Western blot analysis showed that tissue extracts reacted with the anti-UCN, -CRHR1 and –CRHR2 antibodies. RT-PCR experiments demonstrated that mRNAs of UCN, CRHR1 and CRHR2 were expressed. UCN-immunoreactivity (IR) and CRHR2–IR were found in the thyroid follicular and parafollicular cells and CRHR1-IR in the smooth muscle of the blood vessels. These results suggest that a regulatory system exists in the bovine thyroid gland based on UCN, CRHR1 and CRHR2 and that UCN plays a role in the regulation of thyroid physiological functions through an autocrine/paracrine mechanism

Presence and distribution of urocortin and corticotrophin-releasing hormone receptors in the bovine thyroid gland / Squillacioti, Caterina; DE LUCA, Adriana; S., Alì; Ciarcia, Roberto; Germano, Giuseppe; Vittoria, Alfredo; Mirabella, Nicola. - In: ANATOMIA HISTOLOGIA EMBRYOLOGIA. - ISSN 0340-2096. - 43:6(2014), pp. 429-434. [10.1111/ahe.12094]

Presence and distribution of urocortin and corticotrophin-releasing hormone receptors in the bovine thyroid gland

SQUILLACIOTI, CATERINA;DE LUCA, ADRIANA;CIARCIA, ROBERTO;GERMANO, GIUSEPPE;VITTORIA, ALFREDO;MIRABELLA, NICOLA
2014

Abstract

Urocortin (UCN), a 40 amino acid peptide is a Corticotrophin-Releasing Hormone (CRH) related peptide. The biological actions of CRH family peptides are mediated via two types of G-protein coupled receptors, CRH type 1 (CRHR1) and CRH type 2 (CRHR2). The aim of the present study was to investigate the expression of UCN, CRHR1 and CRHR2 by immunoprecipitation, Western blot, immunohistochemistry and RT-PCR in the bovine thyroid gland. Immunoprecipitation and Western blot analysis showed that tissue extracts reacted with the anti-UCN, -CRHR1 and –CRHR2 antibodies. RT-PCR experiments demonstrated that mRNAs of UCN, CRHR1 and CRHR2 were expressed. UCN-immunoreactivity (IR) and CRHR2–IR were found in the thyroid follicular and parafollicular cells and CRHR1-IR in the smooth muscle of the blood vessels. These results suggest that a regulatory system exists in the bovine thyroid gland based on UCN, CRHR1 and CRHR2 and that UCN plays a role in the regulation of thyroid physiological functions through an autocrine/paracrine mechanism
2014
Presence and distribution of urocortin and corticotrophin-releasing hormone receptors in the bovine thyroid gland / Squillacioti, Caterina; DE LUCA, Adriana; S., Alì; Ciarcia, Roberto; Germano, Giuseppe; Vittoria, Alfredo; Mirabella, Nicola. - In: ANATOMIA HISTOLOGIA EMBRYOLOGIA. - ISSN 0340-2096. - 43:6(2014), pp. 429-434. [10.1111/ahe.12094]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/562656
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