Raman microspectroscopy proved to be a valuable support to protein crystallography in all the steps of 3D structure determination. Authors have been using this approach to study changes in chemical composition [1, 2, 3], in secondary [3,4] and tertiary structure [5], and to follow chemical mechanisms [3, 6, 7]. The binding properties of AziRu (Fig. 1), a Ru(III) complex with high antiproliferative activity, toward hen egg white lysozyme [7], RNase A and human serum albumin have been investigated by X-ray crystallography and Raman microspectroscopy. The data provide clues on the mechanism of AziRu-protein adduct formation and clear evidences of ligand exchange in the crystal state (Figs. 2). The combined use of Raman and X-ray crystallography has provided complementary information that a single technique alone would not produce. For instance, in the case of hen egg white lysozyme [7], Raman spectra show not only the ligand exchange that is apparent in the X-ray structures but also the presence of Ru−O−Ru bonds (Fig. 3), which are not revealed by crystallography. References [1] Vergara, et al., Acta Cryst. Sect D: Biol Crystallogr. 64, 167-171 (2008). [2] Caterino et al., A. Vergara , J. Sol. Chem., in press. [3] Merlino, et al. Vergara, A., Acta Cryst. Sect D: Biol Crystallogr , 69, 137-40 (2013). [4] Merlino, et al. A. Vergara, Biophys. Chem. 137, 24-27 (2008). [5] Vergara, et al., J. Biol. Chem. 285, 32568-32575 (2010) . [6] Vitagliano, L., Vergara, et al.., J Am Chem Soc. 130, 10527-10535 (2008). [7] Vergara, et al, Inorg Chem 52, 4157-9 (2013).

RUTHENIUM-BASED ANTICANCER ACTIVITY: A RAMAN-ASSISTED X-RAY CRYSTALLOGRAPHY APPROACH / Vergara, Alessandro; RUSSO KRAUSS, Irene; D'Errico, Gerardino; G., Mangiapia; Montesarchio, Daniela; Paduano, Luigi; Merlino, Antonello. - ELETTRONICO. - (2013), pp. 91-91. (Intervento presentato al convegno European Conference on the Spectroscopy of Biological Molecules (ECSBM15) tenutosi a St Catherine's College, Oxford, UK nel 25 - 30 Agosto 2013).

RUTHENIUM-BASED ANTICANCER ACTIVITY: A RAMAN-ASSISTED X-RAY CRYSTALLOGRAPHY APPROACH

VERGARA, ALESSANDRO;RUSSO KRAUSS, IRENE;D'ERRICO, GERARDINO;MONTESARCHIO, DANIELA;PADUANO, LUIGI;MERLINO, ANTONELLO
2013

Abstract

Raman microspectroscopy proved to be a valuable support to protein crystallography in all the steps of 3D structure determination. Authors have been using this approach to study changes in chemical composition [1, 2, 3], in secondary [3,4] and tertiary structure [5], and to follow chemical mechanisms [3, 6, 7]. The binding properties of AziRu (Fig. 1), a Ru(III) complex with high antiproliferative activity, toward hen egg white lysozyme [7], RNase A and human serum albumin have been investigated by X-ray crystallography and Raman microspectroscopy. The data provide clues on the mechanism of AziRu-protein adduct formation and clear evidences of ligand exchange in the crystal state (Figs. 2). The combined use of Raman and X-ray crystallography has provided complementary information that a single technique alone would not produce. For instance, in the case of hen egg white lysozyme [7], Raman spectra show not only the ligand exchange that is apparent in the X-ray structures but also the presence of Ru−O−Ru bonds (Fig. 3), which are not revealed by crystallography. References [1] Vergara, et al., Acta Cryst. Sect D: Biol Crystallogr. 64, 167-171 (2008). [2] Caterino et al., A. Vergara , J. Sol. Chem., in press. [3] Merlino, et al. Vergara, A., Acta Cryst. Sect D: Biol Crystallogr , 69, 137-40 (2013). [4] Merlino, et al. A. Vergara, Biophys. Chem. 137, 24-27 (2008). [5] Vergara, et al., J. Biol. Chem. 285, 32568-32575 (2010) . [6] Vitagliano, L., Vergara, et al.., J Am Chem Soc. 130, 10527-10535 (2008). [7] Vergara, et al, Inorg Chem 52, 4157-9 (2013).
2013
RUTHENIUM-BASED ANTICANCER ACTIVITY: A RAMAN-ASSISTED X-RAY CRYSTALLOGRAPHY APPROACH / Vergara, Alessandro; RUSSO KRAUSS, Irene; D'Errico, Gerardino; G., Mangiapia; Montesarchio, Daniela; Paduano, Luigi; Merlino, Antonello. - ELETTRONICO. - (2013), pp. 91-91. (Intervento presentato al convegno European Conference on the Spectroscopy of Biological Molecules (ECSBM15) tenutosi a St Catherine's College, Oxford, UK nel 25 - 30 Agosto 2013).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/562395
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