Cysteine-containing antimicrobial peptides of diverse phylogeny share a common structural signature, the γ core, characterized by a strong polarization of charges in two antiparallel β sheets. In this work, we analyzed peptides derived from the tomato defensin SolyC07g007760 corresponding to the protein γ core and demonstrated that cyclization of the peptides, which results in segregation of positive charges to the turn region, produces peptides very active against Gram negative bacteria, such as Salmonella enterica and Helicobacter pylori. Interestingly, these peptides show very low hemolytic activity and thus represent a scaffold for the design of new antimicrobial peptides.

Antimicrobial peptides from plants: stabilization of the core of a tomato defensin by intramolecular disulfide bond / Avitabile, Concetta; Capparelli, Rosanna; Rigano, MARIA MANUELA; Fulgione, Andrea; Barone, Amalia; Pedone, Carlo; Romanelli, Alessandra. - In: JOURNAL OF PEPTIDE SCIENCE. - ISSN 1075-2617. - 19:4(2013), pp. 240-245. [10.1002/psc.2479]

Antimicrobial peptides from plants: stabilization of the core of a tomato defensin by intramolecular disulfide bond

AVITABILE, CONCETTA;CAPPARELLI, ROSANNA;RIGANO, MARIA MANUELA;FULGIONE, ANDREA;BARONE, AMALIA;PEDONE, CARLO;ROMANELLI, ALESSANDRA
2013

Abstract

Cysteine-containing antimicrobial peptides of diverse phylogeny share a common structural signature, the γ core, characterized by a strong polarization of charges in two antiparallel β sheets. In this work, we analyzed peptides derived from the tomato defensin SolyC07g007760 corresponding to the protein γ core and demonstrated that cyclization of the peptides, which results in segregation of positive charges to the turn region, produces peptides very active against Gram negative bacteria, such as Salmonella enterica and Helicobacter pylori. Interestingly, these peptides show very low hemolytic activity and thus represent a scaffold for the design of new antimicrobial peptides.
2013
Antimicrobial peptides from plants: stabilization of the core of a tomato defensin by intramolecular disulfide bond / Avitabile, Concetta; Capparelli, Rosanna; Rigano, MARIA MANUELA; Fulgione, Andrea; Barone, Amalia; Pedone, Carlo; Romanelli, Alessandra. - In: JOURNAL OF PEPTIDE SCIENCE. - ISSN 1075-2617. - 19:4(2013), pp. 240-245. [10.1002/psc.2479]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/548118
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