AIMS: The purpose of this study was to evaluate the antimicrobial efficacy of five different proteases belonging to two different families on Staphylococcus aureus and Staphylococcus epidermidis strains. METHODS AND RESULTS: We used three serine proteases and two metalloproteases in single species biofilm formation assays and in human cell invasion processes. Following each protease incubation with bacterial cells, surface protein patterns were analysed by SDS-PAGE and zymography. Some differently expressed proteins were identified by mass spectrometry. CONCLUSIONS: The effect of tested proteases on biofilm formation was not related to the protease category but was strain-dependent and was related to the biofilm formation capacity of each staphylococcal strain. Some proteases showed a nonspecific and indiscriminate effect on surface proteins, while others induced a discrete and reproducible action on protein profiles. SIGNIFICANCE AND IMPACT OF THE STUDY: The inhibition of the surface-related virulence factors is a promising avenue to overcome persistent infections caused by bacterial biofilms. To this end, we show here that proteases, in particular the metalloprotease serratiopeptidase, can interfere with adhesion and invasion of eukaryotic cells and biofilm formation in staphylococci and their use could represent a viable treatment for the development of novel combination therapies

Comparison of the action of different proteases on virulence properties related to the staphylococcal surface / M., Artini; R., Papa; G. L., Scoarughi; Galano, Eugenio; G., Barbato; Pucci, Pietro; L., Selan. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - STAMPA. - 114:1(2013), pp. 266-277. [10.1111/jam.12038]

Comparison of the action of different proteases on virulence properties related to the staphylococcal surface.

GALANO, EUGENIO;PUCCI, PIETRO;
2013

Abstract

AIMS: The purpose of this study was to evaluate the antimicrobial efficacy of five different proteases belonging to two different families on Staphylococcus aureus and Staphylococcus epidermidis strains. METHODS AND RESULTS: We used three serine proteases and two metalloproteases in single species biofilm formation assays and in human cell invasion processes. Following each protease incubation with bacterial cells, surface protein patterns were analysed by SDS-PAGE and zymography. Some differently expressed proteins were identified by mass spectrometry. CONCLUSIONS: The effect of tested proteases on biofilm formation was not related to the protease category but was strain-dependent and was related to the biofilm formation capacity of each staphylococcal strain. Some proteases showed a nonspecific and indiscriminate effect on surface proteins, while others induced a discrete and reproducible action on protein profiles. SIGNIFICANCE AND IMPACT OF THE STUDY: The inhibition of the surface-related virulence factors is a promising avenue to overcome persistent infections caused by bacterial biofilms. To this end, we show here that proteases, in particular the metalloprotease serratiopeptidase, can interfere with adhesion and invasion of eukaryotic cells and biofilm formation in staphylococci and their use could represent a viable treatment for the development of novel combination therapies
2013
Comparison of the action of different proteases on virulence properties related to the staphylococcal surface / M., Artini; R., Papa; G. L., Scoarughi; Galano, Eugenio; G., Barbato; Pucci, Pietro; L., Selan. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - STAMPA. - 114:1(2013), pp. 266-277. [10.1111/jam.12038]
File in questo prodotto:
File Dimensione Formato  
DifferentProteasesJAppMicrobiology2013.pdf

accesso aperto

Descrizione: Articolo Principale
Tipologia: Documento in Post-print
Licenza: Dominio pubblico
Dimensione 446.43 kB
Formato Adobe PDF
446.43 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/533059
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 34
social impact