X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an additional experimental source of denitrosylation. The system under investigation is the Hb from an Antarctic fish, Trematomus bernacchii (HbTb). To the best of our knowledge, this is the first Raman-assisted crystallographic evidence of X-ray induced NO-photodissociation at a 3rd generation synchrotron (beamline X10SA of the Swiss Light Source).
Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study / Merlino, A., M. R., F., A., B., Pica, A., F., D., G., P., L., M., Vergara, A.. - ELETTRONICO. - (2012), pp. 15-15. (3rd Workshop on the Simultaneous Combination of Spectroscopies with X-ray Absorption, Scattering and Diffraction Techniques Zurich July 4 – 6, 2012).
Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study
MERLINO, ANTONELLO;PICA, ANDREA;VERGARA, ALESSANDRO
2012
Abstract
X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an additional experimental source of denitrosylation. The system under investigation is the Hb from an Antarctic fish, Trematomus bernacchii (HbTb). To the best of our knowledge, this is the first Raman-assisted crystallographic evidence of X-ray induced NO-photodissociation at a 3rd generation synchrotron (beamline X10SA of the Swiss Light Source).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
