EFFECTS OF NDL-PCB AND TCDD ON INTESTINAL EPITHELIAL CELLS HOMEOSTASIS

Polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins (PCDD) are persistent organic pollutants (POPs) recognized as causing adverse effects on humans, animals and environment. Exposure occurs mainly through the consumption of contaminated food, in particular those of animal origin. the aim of the current study was to evaluate the effects of three ndl-PCB congeners, PCB 138, PCB 153 and PCB 180, and tetrachlorodibenzo-p-dioxins (TCDD), alone and differently combined, on non tumorigenic rat intestinal epithelial cell line (IEC-6). The results of the current study showed that ndl-PCBs and TCDD reduced significantly cell viability only at the highest concentrations (50-100 µM and 0.1-1 µM, respectively); such effect was not linked to apoptosis induction or cell cycle arrest. The contemporary presence of more than one contaminant (differently combined) did not induce any enhancement of effects on IEC-6 cell line. Intestinal restitution was not affected by low non cytotoxic concentrations of ndl-PCBs and TCDD. The results of the current study highlight the need to continue the evaluation of toxic properties of ndl-PCBs, which represent a less studied PBCs; such studies could provide useful information in particular in term of risk assessment.