A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P212121, with unit-cell parameters a= 76.77, b= 120.68, c= 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.
Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase / A., Ruggiero; Ruocco, MARIA ROSARIA; P., Grimaldi; Arcari, Paolo; M., Masullo; A., Zagari; L., Vitagliano. - In: ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. - ISSN 1744-3091. - STAMPA. - 61:(2005), pp. 606-609. [10.1107/S1744309105027995]
Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase
RUOCCO, MARIA ROSARIA;ARCARI, PAOLO;
2005
Abstract
A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P212121, with unit-cell parameters a= 76.77, b= 120.68, c= 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
