The development of stratified retinal cell architecture is highly conserved in all vertebrates, implying that a common fundamental molecular mechanism is involved in the generation of the organized retina. However, the detailed molecular mechanisms of retinal development are not fully understood. Here we have identified the Xenopus ortholog of prune and show that it is expressed in both differentiating and differentiated retinal domains during development. Interestingly, these spatial and temporal expression patterns coincide with the expression of prune binding partners, the NM23 family members. Overexpression of prune in retinal precur- sor cells significantly increases the ratio of Müller glial cells as observed by modulation of NM23 activity (Mochizuki et al., 2009). However, a mutated form of prune that has replacement of four aspartate (D) res- idues (D'Angelo et al., 2004), essential for phosphodiesterase activity, does not exhibit gliogenic activity. Our observations suggest that Xenopus prune may regulate Müller gliogenesis through phosphodiesterase- mediated regulation of NM23 family members.

Spatial and temporal expressions of prune reveal a role in Müller gliogenesis during Xenopus retinal development / Bilitou, A; De Marco, N; Bello, Am; Garzia, L; Carotenuto, P; Kim, M; Campanella, C; Ohnuma, S; Zollo, Massimo. - In: GENE. - ISSN 0378-1119. - 509:1(2012), pp. 93-103. [10.1016/j.gene.2012.08.001]

Spatial and temporal expressions of prune reveal a role in Müller gliogenesis during Xenopus retinal development

Carotenuto P;ZOLLO, MASSIMO
2012

Abstract

The development of stratified retinal cell architecture is highly conserved in all vertebrates, implying that a common fundamental molecular mechanism is involved in the generation of the organized retina. However, the detailed molecular mechanisms of retinal development are not fully understood. Here we have identified the Xenopus ortholog of prune and show that it is expressed in both differentiating and differentiated retinal domains during development. Interestingly, these spatial and temporal expression patterns coincide with the expression of prune binding partners, the NM23 family members. Overexpression of prune in retinal precur- sor cells significantly increases the ratio of Müller glial cells as observed by modulation of NM23 activity (Mochizuki et al., 2009). However, a mutated form of prune that has replacement of four aspartate (D) res- idues (D'Angelo et al., 2004), essential for phosphodiesterase activity, does not exhibit gliogenic activity. Our observations suggest that Xenopus prune may regulate Müller gliogenesis through phosphodiesterase- mediated regulation of NM23 family members.
2012
Spatial and temporal expressions of prune reveal a role in Müller gliogenesis during Xenopus retinal development / Bilitou, A; De Marco, N; Bello, Am; Garzia, L; Carotenuto, P; Kim, M; Campanella, C; Ohnuma, S; Zollo, Massimo. - In: GENE. - ISSN 0378-1119. - 509:1(2012), pp. 93-103. [10.1016/j.gene.2012.08.001]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/512680
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