We explored the use of porous silicon (pSi) technology for the construction of a biotechnological device, in which the ligand-receptor interactions are revealed by means of laser optical measurements. Here we report the settling of chemical procedures for the functionalization of the silicon wafers and for the subsequent anchoring of biological molecules such as a purified murine monoclonal antibody (UN1 mAb), an antibody anti-P8 protein of M13 phage and an antibody anti-A20 murine lymphoma cell line. The optical analysis of the interaction on the biochips between the bound biomolecules and their corresponding ligands indicated that the pSi is suitable for this
The ligand-receptor interactions based on silicon technology / Lamberti, Annalisa; C., Sanges; L., Rotiroti; E., De Tommasi; L., De Stefano; G., Scala; I., Rendina; Arcari, Paolo. - In: CURRENT TOPICS IN BIOTECHNOLOGY. - ISSN 0972-821X. - 5:(2009), pp. 49-54.
The ligand-receptor interactions based on silicon technology
LAMBERTI, ANNALISA;ARCARI, PAOLO
2009
Abstract
We explored the use of porous silicon (pSi) technology for the construction of a biotechnological device, in which the ligand-receptor interactions are revealed by means of laser optical measurements. Here we report the settling of chemical procedures for the functionalization of the silicon wafers and for the subsequent anchoring of biological molecules such as a purified murine monoclonal antibody (UN1 mAb), an antibody anti-P8 protein of M13 phage and an antibody anti-A20 murine lymphoma cell line. The optical analysis of the interaction on the biochips between the bound biomolecules and their corresponding ligands indicated that the pSi is suitable for this| File | Dimensione | Formato | |
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