In this work, we have fabricated a porous silicon (PSi) Bragg reflectors microarray using a proper technological process based on photolithography and electrochemical anodization of silicon. Each element of the array is characterized by a diameter of 200 μm. The PSi structures have been used as platform to immobilize label-free DNA probe and a simple optical method has been employed to investigate the interaction between probe-DNA and its complementary target. In order to confirm the specificity of the DNA hybridization, we have also verified that the reaction of probe-DNA with non-complementary DNA did not occur.

A porous silicon based microarray for label-free optical detection of DNA hybridization

LAMBERTI, ANNALISA;
2010

Abstract

In this work, we have fabricated a porous silicon (PSi) Bragg reflectors microarray using a proper technological process based on photolithography and electrochemical anodization of silicon. Each element of the array is characterized by a diameter of 200 μm. The PSi structures have been used as platform to immobilize label-free DNA probe and a simple optical method has been employed to investigate the interaction between probe-DNA and its complementary target. In order to confirm the specificity of the DNA hybridization, we have also verified that the reaction of probe-DNA with non-complementary DNA did not occur.
9780819480026
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/511452
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