The principal components involved in the transcription process in Archaea have been identified; however results about regulation of the expression of archaeal genes at the level of transcription are preliminary. In order to elucidate mechanisms controlling gene expression, we analysed the in vivo expression of the alcohol dehydrogenase gene (adh) in a new isolate of Sulfolobus solfataricus. The location of the transcription initiation site and the TATA box motif were determined and found to match the consensus sequence from different archaeal promoter regions. Comparison of other adh promoters belonging to different related Sulfolobus species revealed the presence of conserved putative regulatory sequence elements in the 5' region immediately flanking the TATA element. Northern blot analysis and enzymatic assays demonstrated that the transcription and the expression of the Ssadh gene is growth phase regulated and responds to different compounds in the medium. The potential to identify regulatory sequences by in vivo mutational analysis using a plasmid based reporter system will be discussed.
The substrate effect and the growth phase dependent expression of the alcohol dehydrogenase from Sulfolobus solfataricus / Fiorentino, Gabriella; R., Cannio; M., Rossi; Bartolucci, Simonetta. - STAMPA. - (1998), pp. ---. (Intervento presentato al convegno European Cell Factory Workshop on Vector construction for Hyperthermophilic Archaea tenutosi a Gif sur Yvette, France nel november 9-10).
The substrate effect and the growth phase dependent expression of the alcohol dehydrogenase from Sulfolobus solfataricus.
FIORENTINO, GABRIELLA;BARTOLUCCI, SIMONETTA
1998
Abstract
The principal components involved in the transcription process in Archaea have been identified; however results about regulation of the expression of archaeal genes at the level of transcription are preliminary. In order to elucidate mechanisms controlling gene expression, we analysed the in vivo expression of the alcohol dehydrogenase gene (adh) in a new isolate of Sulfolobus solfataricus. The location of the transcription initiation site and the TATA box motif were determined and found to match the consensus sequence from different archaeal promoter regions. Comparison of other adh promoters belonging to different related Sulfolobus species revealed the presence of conserved putative regulatory sequence elements in the 5' region immediately flanking the TATA element. Northern blot analysis and enzymatic assays demonstrated that the transcription and the expression of the Ssadh gene is growth phase regulated and responds to different compounds in the medium. The potential to identify regulatory sequences by in vivo mutational analysis using a plasmid based reporter system will be discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
