β-Galactosidase was immobilized on a nylonmembrane chemically grafted with butylmethacrylate. Hexamethylenediamine was used as spacer and glutaraldehyde as coupling agent. The catalytic behaviour of the membrane was studied under isothermal and non-isothermalconditions. It was found that in the presence of temperature gradients, the catalytic membrane exhibited higher activity and greater affinity for the substrate. The increases of the enzyme reaction rate was found to be proportional to the intensity of the applied temperature gradient. Percentage increases of the activity were also found to decrease with the increase of the average temperature and concentration. All the results are phenomenologically discussed in terms of substrate traffic across the catalytic membrane induced by the processes of diffusion or thermodialysis under isothermal and non-isothermalconditions, respectively.
Influence of the non-isothermal conditions on the activity of enzymes immobilized on nylon grafted membranes / M. M., El Masry; DE MAIO, Anna; S., Di Martino; V., Grano; S., Rossi; N., Pagliuca; Z. H., Abd El Latifa; A. B., Moustafa; A., D’Uva; F. S., Gaeta; D. G., Mita. - In: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC. - ISSN 1381-1177. - ELETTRONICO. - 11:(2000), pp. 113-126. [10.1016/S1381-1177(00)00220-4]
Influence of the non-isothermal conditions on the activity of enzymes immobilized on nylon grafted membranes
DE MAIO, ANNA;
2000
Abstract
β-Galactosidase was immobilized on a nylonmembrane chemically grafted with butylmethacrylate. Hexamethylenediamine was used as spacer and glutaraldehyde as coupling agent. The catalytic behaviour of the membrane was studied under isothermal and non-isothermalconditions. It was found that in the presence of temperature gradients, the catalytic membrane exhibited higher activity and greater affinity for the substrate. The increases of the enzyme reaction rate was found to be proportional to the intensity of the applied temperature gradient. Percentage increases of the activity were also found to decrease with the increase of the average temperature and concentration. All the results are phenomenologically discussed in terms of substrate traffic across the catalytic membrane induced by the processes of diffusion or thermodialysis under isothermal and non-isothermalconditions, respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
