Integrin binding to immobilized collagen and fibronectin stimulates the proliferation of human thyroid cells in culture

The expression of integrins of the beta(1) family and their possible biological effects were investigated in normal human thyroid cells in monolayer culture. The expression of beta(1) and alpha(1-6) integrin subunits was determined by flow cytofluorometry with specific antibodies. Follicular cells of subconfluent monolayer cultures expressed alpha(2) beta(1) and alpha(3) beta(1) at high levels, while alpha(1) beta(1) was only slightly expressed, and alpha(4) beta(1), alpha(5) beta(1) and alpha(6) beta(1) were never detected. Cell attachment assays were performed in fibronectin-, type I collagen-, and laminin-coated microtiter plates. Thyroid cells, while adherent to collagen and fibronectin, showed poor attachment to laminin despite the abundance of their putative receptors alpha(2) beta(1) and alpha(3) beta(1). In serum-free medium, collagen and fibronectin induced cytoskeletal organization, change of cell shape ii om round to flat, and cell spreading. [H-3]Thymidine incorporation and proliferation assays were used to evaluate the effects of collagen and fibronectin on DNA synthesis and cell growth in the absence of a change in spreading or cell shape. Both substrates, in low serum containing medium, induced a concentration-dependent increase in [H-3]thymidine incorporation partially inhibited by RGD-containing peptides that blocked the cell attachment.