Agrobacterium tumefaciens strain A281 carrying plasmid pGA472 was used to stably transform eggplant cotyledonary tissue to kanamycin resistance. Both presence and expression of the neo transgene were demonstrated by DNA dot blot analysis and NPT II enzyme assays. Kanamycin-resistant plantlets were regenerated by organogenesis after six months of culture.
Use of hypervirulent Agrobacterium tumefaciens carrying a binary vector to transform eggplant (Solanum melongena) cotyledonary leaf discs / Filippone, Edgardo; Lurquin, P. F.. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - STAMPA. - 280:(1990), pp. 523-526.
Use of hypervirulent Agrobacterium tumefaciens carrying a binary vector to transform eggplant (Solanum melongena) cotyledonary leaf discs
FILIPPONE, EDGARDO;
1990
Abstract
Agrobacterium tumefaciens strain A281 carrying plasmid pGA472 was used to stably transform eggplant cotyledonary tissue to kanamycin resistance. Both presence and expression of the neo transgene were demonstrated by DNA dot blot analysis and NPT II enzyme assays. Kanamycin-resistant plantlets were regenerated by organogenesis after six months of culture.File in questo prodotto:
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