I valori ematologici e le cellule del sangue delle razze

The AA., on the blood taken from the conus arteriosus of ten specimens of Rajas, have determinated the following hematological average values: hemoglobin (Hgb) 2.75 g/100 ml; hema¬tocrite (Ht) 19.25%; red cells 162860/pl; leukocytes 35000/41; M.C.V. 1554; M.C.H. 255; M.C.H.C. 14.16. The following cytochemical methods have been performed: benzidine-peroxida¬se after Lepehne (for Hgb); sudan black B (for hpids); PAS (for neutral polysaccharides); alcian blue at pH 1.0 and 2.5 (for acid polysaccharides); safranine 0 (1:1000) and toluidine blue (1:100) (for metachromasia); Hg-bromophenol blue and fast green (for proteins); :,'Aenosinetriphosphatase (ATPase), acid phosphatase (ACP), alkaline phosphatase (ALP), aliesteras, +:ALE) and peroxidase (PEO) (for enzymes). Of the red cells, three types have been described (Fig. 1): the mature erythrocytes (QJ 25.43 x 15.79 4m), the acidophilic erythroblastes (f0 19.08 x 12.91 4m) and the basophilic erythrobla¬stes (0 14.84). The first is ellipsoidal in shape and strongly positive to benzidine-peroxidase reaction; the second (7% of all red cells) is ellipsoidal in shape and moderately positive to benzidine¬peroxidase (a 5%ro is in mitosis); the third (3%ro of all red cells) is round in shape and weakly positive to benzidine-peroxidase (a 10°'o is in mitosis). Of the leukocytes, five types have been described: lymphocytes, monocytes, neutrophilic granulocytes, eosinophilic granulocytes, and thrombocytes. The lymphocytes (65.66% in the quali¬tative count; 0 10.60 4m) present a scanty cytoplasma and a big round nucleus. The cytoplasm is light blue with M.G.G. (Fig. 2 e 3) and weakly positive to PAS method and sometimes contains a few violet granules weakly positive to ALE and ACP. The monocytes (Fig. 2) (2.38% in the qualitative count; 0 14.66 x 13.56 4m) round in shape, present a large cytoplasm and a horseshoe nucleus. The cytoplasm contains a few little violet granules positive to PAS and ACP (Fig. 7) and the ground positive to ALE. The neutrophilic granulocytes (21.31% in the qualitative count; 0 16.80 4m) round in shape, show a large cytoplasm and either a round (70%) or indented (25%) or lobulated (5%) nucleus. They contain elongated or grain rice-shaped granoles (1.0 x 0.3 4m) pale grey-blue or pale pink with M.G.G. (Fig. 3), positive to ATPase (Fig. 8). T- e cytoplasmic ground is PAS (Fig. 5), ACP (Fig. 7), and ALE (Fig. 6) positive. The eosinophilic granulocytes (10.65% in the qualitative count; 0 15.09 4m) round in shape, present a cytoplasm rich in round granules (2.50 4m O) strongly eosinophilic with M.G.G. Fig. 4) and positive to Hg-bromophenol blue and fast green. The cytoplasmic ground is PAS positive (Fig. 5), weakly positive to ALE (Fig. 6) and strongly positive to ACP (Fig. 7). The thrombocytes (18900/41') either round and a little elongated (58% of the total number; Q` 9.38 4m) or spindle-shaped (25°ío of the total number; ,0 15.06 x 9.32 4m) (besides a 17% of transitional forms), show the cytoplasm light blue with M.G.G. (Fig. 2) with fine violet granules PAS (Fig. 5) and ALE positive and with ATPase activity (Fig. 8). In conclusion the red cells have been distinguished in basophilic erythroblastes, acidophilic erythroblastes and mature erythrocytes. As regards the leukocytes it has been taken into account the cytochemical homology between the leukocytes of Rajas, those of Torpedoes and the human ones, therefore the distinction in neutrophilic and eosinophilic granulocytes results to be exact. For this reason the name of heterophil cannot be applied to any blood cells of Torpedoes and Rajas.