In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, corresponding to several classes of homeodomains, have been identified. Five of the isolated homeoboxes show their highest homology to members of the Vertebrate HOX clusters. mRNAs for two of the isolated homeoboxes are present in unfertilized C. intestinalis eggs
Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster / Di Gregorio, A; Spagnuolo, A; Ristoratore, F; Pischetola, M; Aniello, Francesco; Branno, M; Cariello, L; DI LAURO, Roberto. - In: GENE. - ISSN 0378-1119. - ELETTRONICO. - 156:(1995), pp. 253-257.
Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster
ANIELLO, FRANCESCO;DI LAURO, ROBERTO
1995
Abstract
In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, corresponding to several classes of homeodomains, have been identified. Five of the isolated homeoboxes show their highest homology to members of the Vertebrate HOX clusters. mRNAs for two of the isolated homeoboxes are present in unfertilized C. intestinalis eggsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
