PCR primers, VQ5 and VQ14, were designed from regions. of goat as2-Cn cDNA sequence (GenBank accession no X65160)' corresponding to exons 17 and 18 of bovine as2-Cn gene (GenBank accession no. M943227).' The amplified DNA fragments revealed a polymorphism. Two alleles were observed: the A allele (showing a fragment of about 3300 bp) and the B allele (showing a fragment of about 3700 bp). Frequencies of the two alleles determined in 150 unrelated goats of undefined genetic type reared in Southern Italy were 0.6 (A) and 0.4 (B).
File in questo prodotto:
Non ci sono file associati a questo prodotto.