PCR primers, VQ5 and VQ14, were designed from regions. of goat as2-Cn cDNA sequence (GenBank accession no X65160)' corresponding to exons 17 and 18 of bovine as2-Cn gene (GenBank accession no. M943227).' The amplified DNA fragments revealed a polymorphism. Two alleles were observed: the A allele (showing a fragment of about 3300 bp) and the B allele (showing a fragment of about 3700 bp). Frequencies of the two alleles determined in 150 unrelated goats of undefined genetic type reared in Southern Italy were 0.6 (A) and 0.4 (B).

A PCR polymorphism detected in the goat as2-casein gene

RAMUNNO, LUIGI;COSENZA, GIANFRANCO;
1997

Abstract

PCR primers, VQ5 and VQ14, were designed from regions. of goat as2-Cn cDNA sequence (GenBank accession no X65160)' corresponding to exons 17 and 18 of bovine as2-Cn gene (GenBank accession no. M943227).' The amplified DNA fragments revealed a polymorphism. Two alleles were observed: the A allele (showing a fragment of about 3300 bp) and the B allele (showing a fragment of about 3700 bp). Frequencies of the two alleles determined in 150 unrelated goats of undefined genetic type reared in Southern Italy were 0.6 (A) and 0.4 (B).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/471943
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