A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains (Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F-1 (AKR x MOLF/Ei) and F-1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4(+) lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.
Monoallelic expression of mouse Cd4 gene / Capparelli, Rosanna; A., Costabile; M., Viscardi; D., Iannelli. - In: MAMMALIAN GENOME. - ISSN 0938-8990. - ELETTRONICO. - 15:(2004), pp. 579-584. [10.1007/s00335-004-2351-y]
Monoallelic expression of mouse Cd4 gene
CAPPARELLI, ROSANNA;
2004
Abstract
A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains (Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F-1 (AKR x MOLF/Ei) and F-1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4(+) lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
