1. BRL-3A cells posses a specific LDL receptor with an apparent mol. wt of 160,000 that binds, with saturation, both human and rat I-125-LDL. 2. Like human fibroblasts, BRL-3A cells also bind, internalize and degrade I-125-hLDL but to a lesser extent. 3. BRL-3A cells also bind the monoclonal antibody against rat liver LDL receptor P1B3. Moreover the LDL receptor activity increases when cells are preincubated with medium containing 5% of LPDS. 4. As with human (h) fibroblasts, treatment of BRL-3A cells with 10(-7) M insulin enhances binding (30%), internalization (18%) and degradation (20%) of I-125-hLDL.
Characterization of the Low-density-lipoprotein Receptor Activity In Buffalo Rat-liver (brl-3a) Cells / Santillo, Mariarosaria; Mondola, Paolo; Santangelo, Franco; Belfiore, Anna; Tedesco, I.; Laezza, C.; Cerbone, R.; Romano, A.; Bifulco, M.. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. - ISSN 0305-0491. - STAMPA. - 101:(1992), pp. 489-494. [10.1016/0305-0491(92)90032-M]
Characterization of the Low-density-lipoprotein Receptor Activity In Buffalo Rat-liver (brl-3a) Cells
SANTILLO, MARIAROSARIA;MONDOLA, PAOLO;SANTANGELO, FRANCO;BELFIORE, ANNA;M. BIFULCO
1992
Abstract
1. BRL-3A cells posses a specific LDL receptor with an apparent mol. wt of 160,000 that binds, with saturation, both human and rat I-125-LDL. 2. Like human fibroblasts, BRL-3A cells also bind, internalize and degrade I-125-hLDL but to a lesser extent. 3. BRL-3A cells also bind the monoclonal antibody against rat liver LDL receptor P1B3. Moreover the LDL receptor activity increases when cells are preincubated with medium containing 5% of LPDS. 4. As with human (h) fibroblasts, treatment of BRL-3A cells with 10(-7) M insulin enhances binding (30%), internalization (18%) and degradation (20%) of I-125-hLDL.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
