Vascular differentiation involves not only the correct functionality of water transport system, but it also has influence on softness of tissues, food digestibility and consequently on its quality. The aim of this study is to investigate the effect of clinostat rotation on seedling growth and vascular differentiation of soy. Seeds germinated either on an uni-axial clinostat or in 1g under different temperature regimes. Several parameters such as percent germination, length of the radicle, fresh and dry weights, were recorded. Anatomy of cotyledons, hypocotyl hook and hypocotyl was quantified through image analysis software programs. Parameters such as area of vessel lumen, thickness of cell walls, area and shape of parenchyma cells, presence of endodermis, yield and distribution of storage substances were recorded. Results showed that the development of well-structured seedlings was possible in few days both in Ig and in simulated low-gravity. The overall analysis suggests that vascular system in soy seedlings is slightly affected by clinostat rotation. Changes in polyphenols distribution acted as an adaptive response to the stress of altered gravity condition. Moreover, anatomical analysis was a better method to evaluate the state of hydration of seedlings than the overall morphology. Copyright © 2003 by the International Astronautical Federation. All rights reserved.
Effect of simulated microgravity on seedling development and vascular differentiation of soy / DE MICCO, Veronica; Rocco, L.; DE PASCALE, Stefania; Aronne, Giovanna. - 1:(2003), pp. 1371-1381. (Intervento presentato al convegno 54th International Astronautical Congress tenutosi a Bremen, Germany nel 2003).
Effect of simulated microgravity on seedling development and vascular differentiation of soy
DE MICCO, VERONICA;DE PASCALE, STEFANIA;ARONNE, GIOVANNA
2003
Abstract
Vascular differentiation involves not only the correct functionality of water transport system, but it also has influence on softness of tissues, food digestibility and consequently on its quality. The aim of this study is to investigate the effect of clinostat rotation on seedling growth and vascular differentiation of soy. Seeds germinated either on an uni-axial clinostat or in 1g under different temperature regimes. Several parameters such as percent germination, length of the radicle, fresh and dry weights, were recorded. Anatomy of cotyledons, hypocotyl hook and hypocotyl was quantified through image analysis software programs. Parameters such as area of vessel lumen, thickness of cell walls, area and shape of parenchyma cells, presence of endodermis, yield and distribution of storage substances were recorded. Results showed that the development of well-structured seedlings was possible in few days both in Ig and in simulated low-gravity. The overall analysis suggests that vascular system in soy seedlings is slightly affected by clinostat rotation. Changes in polyphenols distribution acted as an adaptive response to the stress of altered gravity condition. Moreover, anatomical analysis was a better method to evaluate the state of hydration of seedlings than the overall morphology. Copyright © 2003 by the International Astronautical Federation. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
