Liposomes with a DSPC/MonY-BN (97/3 molar ratio) composition showed a diameter of 145.5 +/- 31.5 nm and a polydispersity index of 0.20 +/- 0.05. High doxorubicin (Dox) loading was obtained with the remote pH gradient method using citrate as the inner buffer. Specific binding to PC-3 cells of DSPC/MonY-BN liposomes was obtained (2.7% +/- 0.3%, at 37 degrees C), compared with peptide-free DSPC liposomes (1.4% +/- 0.2% at 37 degrees C). Incubation of cells with DSPC/MonY-BN/Dox showed significantly lower cell survival compared with DSPC/Dox-treated cells, in the presence of 100 ng/mL and 300 ng/mL drug amounts, in cytotoxicity experiments. Intravenous treatment of PC-3 xenograft-bearing mice with DSPC/MonY-BN/Dox at 10 mg/kg Dox dose produced higher tumour growth inhibition (60%) compared with nonspecific DSPC/Dox liposomes (36%) relative to control animals. Conclusion: The structural and loading properties of DSPC/MonY-BN liposomes along with the observed in-vitro and in-vivo activity are encouraging for further development of this approach for target-specific cancer chemotherapy.

Peptide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent / Accardo, Antonella; Salsano, G; Morisco, A; Aurilio, M; Parisi, Antonio; Maione, Francesco; Cicala, Carla; Tesauro, Diego; Aloj, L; DE ROSA, Giuseppe; Morelli, Giancarlo. - In: INTERNATIONAL JOURNAL OF NANOMEDICINE. - ISSN 1176-9114. - 7:(2012), pp. 2007-2017. [10.2147/IJN.S29242]

Peptide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent.

ACCARDO, ANTONELLA;PARISI, ANTONIO;MAIONE, FRANCESCO;CICALA, CARLA;TESAURO, DIEGO;DE ROSA, GIUSEPPE;MORELLI, GIANCARLO
2012

Abstract

Liposomes with a DSPC/MonY-BN (97/3 molar ratio) composition showed a diameter of 145.5 +/- 31.5 nm and a polydispersity index of 0.20 +/- 0.05. High doxorubicin (Dox) loading was obtained with the remote pH gradient method using citrate as the inner buffer. Specific binding to PC-3 cells of DSPC/MonY-BN liposomes was obtained (2.7% +/- 0.3%, at 37 degrees C), compared with peptide-free DSPC liposomes (1.4% +/- 0.2% at 37 degrees C). Incubation of cells with DSPC/MonY-BN/Dox showed significantly lower cell survival compared with DSPC/Dox-treated cells, in the presence of 100 ng/mL and 300 ng/mL drug amounts, in cytotoxicity experiments. Intravenous treatment of PC-3 xenograft-bearing mice with DSPC/MonY-BN/Dox at 10 mg/kg Dox dose produced higher tumour growth inhibition (60%) compared with nonspecific DSPC/Dox liposomes (36%) relative to control animals. Conclusion: The structural and loading properties of DSPC/MonY-BN liposomes along with the observed in-vitro and in-vivo activity are encouraging for further development of this approach for target-specific cancer chemotherapy.
2012
Peptide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent / Accardo, Antonella; Salsano, G; Morisco, A; Aurilio, M; Parisi, Antonio; Maione, Francesco; Cicala, Carla; Tesauro, Diego; Aloj, L; DE ROSA, Giuseppe; Morelli, Giancarlo. - In: INTERNATIONAL JOURNAL OF NANOMEDICINE. - ISSN 1176-9114. - 7:(2012), pp. 2007-2017. [10.2147/IJN.S29242]
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Descrizione: Accardo et al. Peptide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent. Int J Nanomedicine. 2012;7:2007-17.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/460122
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