Developmental processes in the ascidian Ciona intestinalis depend on a complex interplay of events including, during metamorphosis, a caspase-dependent apoptosis which is regulated by the nitric oxide (NO)-cGMP signaling pathway. Herein we disclose an alternate NO-mediated signaling pathway during Ciona development which appears to be critically dependent on local redox control. Evidence in support of this conclusion includes: (a) inhibitors of NO synthase (NOS) and scavengers of NO-derived nitrating agents markedly decrease the rate of Ciona metamorphosis; (b) an NO donor or peroxynitrite caused an opposite effect; (c) increased protein nitration is obsd. at larva stage. Integrated proteomic and immunochem. methodologies identified nitrated tyrosine residues in ERK and snail. Overall, these results point to protein nitration as a hitherto overlooked NO-dependent regulatory mechanism in Ciona which is specifically triggered by elevated ROS prodn. during developmental processes.

Protein nitration as footprint of oxidative stress-related nitric oxide signaling pathways in developing Ciona intestinalis / Ercolesi, E.; Tedeschi, G.; Fiore, G.; Negri, A.; Maffioli, E.; D'Ischia, Marco; Palumbo, A.. - In: NITRIC OXIDE. - ISSN 1351-525X. - 27:1(2012), pp. 18-24. [10.1016/j.niox.2012.03.012]

Protein nitration as footprint of oxidative stress-related nitric oxide signaling pathways in developing Ciona intestinalis

D'ISCHIA, MARCO;
2012

Abstract

Developmental processes in the ascidian Ciona intestinalis depend on a complex interplay of events including, during metamorphosis, a caspase-dependent apoptosis which is regulated by the nitric oxide (NO)-cGMP signaling pathway. Herein we disclose an alternate NO-mediated signaling pathway during Ciona development which appears to be critically dependent on local redox control. Evidence in support of this conclusion includes: (a) inhibitors of NO synthase (NOS) and scavengers of NO-derived nitrating agents markedly decrease the rate of Ciona metamorphosis; (b) an NO donor or peroxynitrite caused an opposite effect; (c) increased protein nitration is obsd. at larva stage. Integrated proteomic and immunochem. methodologies identified nitrated tyrosine residues in ERK and snail. Overall, these results point to protein nitration as a hitherto overlooked NO-dependent regulatory mechanism in Ciona which is specifically triggered by elevated ROS prodn. during developmental processes.
2012
Protein nitration as footprint of oxidative stress-related nitric oxide signaling pathways in developing Ciona intestinalis / Ercolesi, E.; Tedeschi, G.; Fiore, G.; Negri, A.; Maffioli, E.; D'Ischia, Marco; Palumbo, A.. - In: NITRIC OXIDE. - ISSN 1351-525X. - 27:1(2012), pp. 18-24. [10.1016/j.niox.2012.03.012]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/457787
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