An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β -glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue. However, the scarcity of α -glycosynthases has so far hampered access to the synthesis of a large class of oligosaccharides of biotechnological interest. We review here a new glycosynthetic methodology for the production of two retaining α -fucosynthases and an α -galactosynthase exploiting β -glycosyl azide derivatives. The general applicability of this approach, which opens new perspectives in the use of azide derivatives for the production of novel α -glycosynthases, is discussed.

Exploitation of beta-glycosyl azides for the preparation of alpha-glycosynthases / Cobucci Ponzano, B.; Zorzetti, C.; Strazzulli, Andrea; Bedini, Emiliano; Corsaro, MARIA MICHELA; Sulzenbacher, G.; Rossi, M.; Moracci, Marco. - In: BIOCATALYSIS AND BIOTRANSFORMATION. - ISSN 1024-2422. - 30:(2012), pp. 288-295. [10.3109/10242422.2012.679814]

Exploitation of beta-glycosyl azides for the preparation of alpha-glycosynthases

STRAZZULLI, ANDREA;BEDINI, EMILIANO;CORSARO, MARIA MICHELA;MORACCI, Marco
2012

Abstract

An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β -glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue. However, the scarcity of α -glycosynthases has so far hampered access to the synthesis of a large class of oligosaccharides of biotechnological interest. We review here a new glycosynthetic methodology for the production of two retaining α -fucosynthases and an α -galactosynthase exploiting β -glycosyl azide derivatives. The general applicability of this approach, which opens new perspectives in the use of azide derivatives for the production of novel α -glycosynthases, is discussed.
2012
Exploitation of beta-glycosyl azides for the preparation of alpha-glycosynthases / Cobucci Ponzano, B.; Zorzetti, C.; Strazzulli, Andrea; Bedini, Emiliano; Corsaro, MARIA MICHELA; Sulzenbacher, G.; Rossi, M.; Moracci, Marco. - In: BIOCATALYSIS AND BIOTRANSFORMATION. - ISSN 1024-2422. - 30:(2012), pp. 288-295. [10.3109/10242422.2012.679814]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/453421
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