aidB is one of the four genes of E. coli that is induced by alkylating agents and regulated by Ada protein. Three genes (ada, alkA, and alkB) encode DNA repair proteins that remove or repair alkylated bases. However, the role of AidB remains unclear despite extensive efforts to determine its function in cells exposed to alkylating agents. The E. coli AidB protein was identified as a component of the protein complex that assembles at strong promoters. We demonstrate that AidB protein preferentially binds to UP elements, AT rich transcription enhancer sequences found upstream of many highly expressed genes, several DNA repair genes, and housekeeping genes. AidB allows efficient transcription from promoters containing an UP element upon exposure to a DNA methylating agent and protects downstream genes from DNA damage. The DNA binding domain is required to target AidB to specific genes preferentially protecting them from alkylation damage. However, deletion of AidB's DNA binding domain does not prevent its antimutagenic activity, instead this deletion appears to allow AidB to function as a cytoplasmic alkylation resistance protein. Our studies identify the role of AidB in alkylating agent exposed cells and suggest a new cellular strategy in which a subset of the genome is preferentially protected from damage by alkylating agents.

Preferential DNA damage prevention by the E. coli AidB gene: a new mechanism for protection of specific genes / Rippa, Valentina; Duilio, Angela; DI PASQUALE, Pamela; Amoresano, Angela; Landini, P.; Volkert, M. R.. - In: DNA REPAIR. - ISSN 1568-7864. - 10:9(2011), pp. 934-941. [10.1016/j.dnarep.2011.06.001]

Preferential DNA damage prevention by the E. coli AidB gene: a new mechanism for protection of specific genes.

RIPPA, VALENTINA;DUILIO, ANGELA;DI PASQUALE, PAMELA;AMORESANO, ANGELA;
2011

Abstract

aidB is one of the four genes of E. coli that is induced by alkylating agents and regulated by Ada protein. Three genes (ada, alkA, and alkB) encode DNA repair proteins that remove or repair alkylated bases. However, the role of AidB remains unclear despite extensive efforts to determine its function in cells exposed to alkylating agents. The E. coli AidB protein was identified as a component of the protein complex that assembles at strong promoters. We demonstrate that AidB protein preferentially binds to UP elements, AT rich transcription enhancer sequences found upstream of many highly expressed genes, several DNA repair genes, and housekeeping genes. AidB allows efficient transcription from promoters containing an UP element upon exposure to a DNA methylating agent and protects downstream genes from DNA damage. The DNA binding domain is required to target AidB to specific genes preferentially protecting them from alkylation damage. However, deletion of AidB's DNA binding domain does not prevent its antimutagenic activity, instead this deletion appears to allow AidB to function as a cytoplasmic alkylation resistance protein. Our studies identify the role of AidB in alkylating agent exposed cells and suggest a new cellular strategy in which a subset of the genome is preferentially protected from damage by alkylating agents.
2011
Preferential DNA damage prevention by the E. coli AidB gene: a new mechanism for protection of specific genes / Rippa, Valentina; Duilio, Angela; DI PASQUALE, Pamela; Amoresano, Angela; Landini, P.; Volkert, M. R.. - In: DNA REPAIR. - ISSN 1568-7864. - 10:9(2011), pp. 934-941. [10.1016/j.dnarep.2011.06.001]
File in questo prodotto:
File Dimensione Formato  
DNA repair 2011.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: Accesso privato/ristretto
Dimensione 651.08 kB
Formato Adobe PDF
651.08 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/430323
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 17
social impact