Unlike adult mammalians and birds, in which the predominant glial elements are astrocytes, the ependymoglia is main in reptiles. In the present study we investigate the modification of the spinal cord ependymoglia in a model of neuronal plasticity as the regeneration of tail in lizard Gekko gecko. The caudotomy induces the growth of regenerating blastema and its differentiation with complete replacement of the amputated segment. A rudimental spinal cord re-grows into regenerated tail. It consists of an ependymal cell tube originating from the ependyma lining the central canal of the rostral spinal cord to the amputation plane, through to create a cellular scaffold that guides and supports the re-growing central axons. The aim of the present study is to investigate the morphological changes of glial elements in the tail spinal cord of lizard with intact tail compared to the relative region committed to innervate the regrowing tail at 5, and 15 days after caudotomy, and the complete regenerated tail. Single and multiple immunohystochemistry were performed for identification of glial fibrillary acid protein (GFAP) coupled to nuclear neuronal marker (NeuN). Intensely labelled GFAP-immunoreactive elements were found during spinal cord regeneration compared to intact tail. Many of these cells were tanycytes since they belong to subependymal layer and to gray matter of ventro and dorso-lateral part of central lumen. Each tanycyte abuts the lumen and extends a single, large processes into gray matter. The ependymal origin and radial arrangement of tanycytes were evident as they originated perpendicularly from the ependymal lumen and coursed parallel each other. These features suggest a changing of role for these cells, very likely involved in the supportive scaffold of the re-growing axons.

EPENDYMOGLIA SPINAL CORD MODIFICATIONIN IN A MODEL OF NEURONAL PLASTICITY / Ferrandino, Ida; R., Imperatore; G., Zampi; R., Favorito; A., Di Nunzio; L., Cristino. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - STAMPA. - 55/suppl.1:(2011), pp. 30-31. [10.4081/ejh.2011.s1]

EPENDYMOGLIA SPINAL CORD MODIFICATIONIN IN A MODEL OF NEURONAL PLASTICITY

FERRANDINO, IDA;
2011

Abstract

Unlike adult mammalians and birds, in which the predominant glial elements are astrocytes, the ependymoglia is main in reptiles. In the present study we investigate the modification of the spinal cord ependymoglia in a model of neuronal plasticity as the regeneration of tail in lizard Gekko gecko. The caudotomy induces the growth of regenerating blastema and its differentiation with complete replacement of the amputated segment. A rudimental spinal cord re-grows into regenerated tail. It consists of an ependymal cell tube originating from the ependyma lining the central canal of the rostral spinal cord to the amputation plane, through to create a cellular scaffold that guides and supports the re-growing central axons. The aim of the present study is to investigate the morphological changes of glial elements in the tail spinal cord of lizard with intact tail compared to the relative region committed to innervate the regrowing tail at 5, and 15 days after caudotomy, and the complete regenerated tail. Single and multiple immunohystochemistry were performed for identification of glial fibrillary acid protein (GFAP) coupled to nuclear neuronal marker (NeuN). Intensely labelled GFAP-immunoreactive elements were found during spinal cord regeneration compared to intact tail. Many of these cells were tanycytes since they belong to subependymal layer and to gray matter of ventro and dorso-lateral part of central lumen. Each tanycyte abuts the lumen and extends a single, large processes into gray matter. The ependymal origin and radial arrangement of tanycytes were evident as they originated perpendicularly from the ependymal lumen and coursed parallel each other. These features suggest a changing of role for these cells, very likely involved in the supportive scaffold of the re-growing axons.
2011
EPENDYMOGLIA SPINAL CORD MODIFICATIONIN IN A MODEL OF NEURONAL PLASTICITY / Ferrandino, Ida; R., Imperatore; G., Zampi; R., Favorito; A., Di Nunzio; L., Cristino. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - STAMPA. - 55/suppl.1:(2011), pp. 30-31. [10.4081/ejh.2011.s1]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/424032
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