One new (1) and three known (2-4) isonitrile diterpenes, isolated from the Caribbean sponge Pseudoaxinella flava, were assayed in human cancer cell lines in vitro using an MTT colorimetric assay and quantitative videomicroscopy. Compounds 1-4 displayed activity for human PC3 prostate apoptosis-sensitive cancer cell lines. Compounds 3 and 4 demonstrated similar growth inhibitory effects for three apoptosis-sensitive and three apoptosis-resistant cancer cell lines. Quantitative videomicroscopy analysis revealed that compounds 1 and 2 exerted their activity through cytotoxic effects, and compounds 3 and 4 through cytostatic effects. These results identify marine diterpene isonitriles as potential lead compounds for anticancer drug discovery.
Evaluation of the Antiproliferative Activity of Diterpene Isonitriles from the Sponge Pseudoaxinella flava in Apoptosis-sensitive and Apoptosis-resistant Cancer Cell Lines / D., Lamoral Theys; Fattorusso, Ernesto; Mangoni, Alfonso; Perinu, Cristina; R., Kiss; Costantino, Valeria. - In: JOURNAL OF NATURAL PRODUCTS. - ISSN 0163-3864. - 74:(2011), pp. 2299-2303. [10.1021/np2005055]
Evaluation of the Antiproliferative Activity of Diterpene Isonitriles from the Sponge Pseudoaxinella flava in Apoptosis-sensitive and Apoptosis-resistant Cancer Cell Lines
FATTORUSSO, ERNESTO;MANGONI, ALFONSO;PERINU, CRISTINA;COSTANTINO, VALERIA
2011
Abstract
One new (1) and three known (2-4) isonitrile diterpenes, isolated from the Caribbean sponge Pseudoaxinella flava, were assayed in human cancer cell lines in vitro using an MTT colorimetric assay and quantitative videomicroscopy. Compounds 1-4 displayed activity for human PC3 prostate apoptosis-sensitive cancer cell lines. Compounds 3 and 4 demonstrated similar growth inhibitory effects for three apoptosis-sensitive and three apoptosis-resistant cancer cell lines. Quantitative videomicroscopy analysis revealed that compounds 1 and 2 exerted their activity through cytotoxic effects, and compounds 3 and 4 through cytostatic effects. These results identify marine diterpene isonitriles as potential lead compounds for anticancer drug discovery.File | Dimensione | Formato | |
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