Purpose: MicroRNAs (miRNAs) are small, non-coding RNA molecules that can be down- or up-regulated in colorectal cancer and have been associated to prognosis and response to treatment. We studied miRNA expression in tumor biopsies of patients with rectal cancer to identify a specific “signature” correlating with pathological complete response (pCR) after neoadjuvant chemo-radiotherapy. Methods and Materials: 38 T3-4/N+ rectal cancer patients received capecitabine-oxaliplatin and radiotherapy followed by surgery. Pathologic response was scored according to the Mandard TRG scale. MiRNA expression was analysed by microarray and confirmed by RT-PCR on frozen biopsies obtained before treatment. The correlation between miRNA expression and TRG, coded as TRG1 (pCR) versus TRG >1 (no pCR), was assessed by methods specifically designed for this study. Results: Microarray analysis selected 14 miRNAs as being differentially expressed in TRG1 patients, and 13 were confirmed by RT-PCR: 11 miRNAs (miR-1183, miR-483-5p, miR-622, miR-125a-3p, miR-1224-5p, miR-188-5p, miR-1471, miR-671-5p, miR-1909*, miR-630, miR-765) were significantly up-regulated in TRG1 patients, 2 (miR-1274b, miR-720) were down-expressed. MiR-622 and miR-630 had a 100% sensitivity and specificity in selecting TRG1 cases. Conclusions: A set of 13 miRNAs is strongly associated with pCR and may represent a specific

A specific miRNA signature correlates with complete pathological response to neoadjuvant chemo-radiotherapy in locally advanced rectal cancer / DELLA VITTORIA SCARPATI, Giuseppina; Falcetta, Francesca; Carlomagno, Chiara; Ubezio, Paolo; Marchini, Sergio; DE STEFANO, Alfonso; Singh Vijay, Kumar; D’Incalci, Maurizio; DE PLACIDO, Sabino; Pepe, Stefano. - In: INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS. - ISSN 0360-3016. - 83:4(2012), pp. 1113-1119. [10.1016/j.ijrobp.2011.09.030]

A specific miRNA signature correlates with complete pathological response to neoadjuvant chemo-radiotherapy in locally advanced rectal cancer

DELLA VITTORIA SCARPATI, GIUSEPPINA;CARLOMAGNO, Chiara;DE STEFANO, ALFONSO;DE PLACIDO, SABINO;
2012

Abstract

Purpose: MicroRNAs (miRNAs) are small, non-coding RNA molecules that can be down- or up-regulated in colorectal cancer and have been associated to prognosis and response to treatment. We studied miRNA expression in tumor biopsies of patients with rectal cancer to identify a specific “signature” correlating with pathological complete response (pCR) after neoadjuvant chemo-radiotherapy. Methods and Materials: 38 T3-4/N+ rectal cancer patients received capecitabine-oxaliplatin and radiotherapy followed by surgery. Pathologic response was scored according to the Mandard TRG scale. MiRNA expression was analysed by microarray and confirmed by RT-PCR on frozen biopsies obtained before treatment. The correlation between miRNA expression and TRG, coded as TRG1 (pCR) versus TRG >1 (no pCR), was assessed by methods specifically designed for this study. Results: Microarray analysis selected 14 miRNAs as being differentially expressed in TRG1 patients, and 13 were confirmed by RT-PCR: 11 miRNAs (miR-1183, miR-483-5p, miR-622, miR-125a-3p, miR-1224-5p, miR-188-5p, miR-1471, miR-671-5p, miR-1909*, miR-630, miR-765) were significantly up-regulated in TRG1 patients, 2 (miR-1274b, miR-720) were down-expressed. MiR-622 and miR-630 had a 100% sensitivity and specificity in selecting TRG1 cases. Conclusions: A set of 13 miRNAs is strongly associated with pCR and may represent a specific
2012
A specific miRNA signature correlates with complete pathological response to neoadjuvant chemo-radiotherapy in locally advanced rectal cancer / DELLA VITTORIA SCARPATI, Giuseppina; Falcetta, Francesca; Carlomagno, Chiara; Ubezio, Paolo; Marchini, Sergio; DE STEFANO, Alfonso; Singh Vijay, Kumar; D’Incalci, Maurizio; DE PLACIDO, Sabino; Pepe, Stefano. - In: INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS. - ISSN 0360-3016. - 83:4(2012), pp. 1113-1119. [10.1016/j.ijrobp.2011.09.030]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/406248
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