Thymosin-beta 4 (T beta 4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of T beta 4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) T beta 4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of T beta 4. (C) 2011 Elsevier Inc. All rights reserved.
In vitro and in vivo pro-angiogenic effects of thymosin-beta 4-derived peptides / Dettin, M., Ghezzo, F., Conconi, M.T., Urbani, L., D'Auria, G., Falcigno, L., Guidolin, D., Nico, B., Ribatti, D., Di Bello, C., Parnigotto, P.P.. - In: CELLULAR IMMUNOLOGY. - ISSN 0008-8749. - 271:2(2011), pp. 299-307. [10.1016/j.cellimm.2011.07.008]
In vitro and in vivo pro-angiogenic effects of thymosin-beta 4-derived peptides
D'AURIA, GABRIELLA;FALCIGNO, LUCIA;
2011
Abstract
Thymosin-beta 4 (T beta 4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of T beta 4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) T beta 4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of T beta 4. (C) 2011 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
