Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. Using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7 allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy / Spadaro, Giuseppe; C., D'Orio; Genovese, Arturo; A., Galeotafiore; C., D'Ambrosio; S., Di Giovanni; Vitale, Monica; Capasso, Mario; V., Lamberti; A., Scaloni; Marone, Gianni; Zambrano, Nicola. - In: JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. - ISSN 1110-7243. - 2011:(2011), pp. 1-10. [10.1155/2011/706746]
Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy
SPADARO, GIUSEPPE;GENOVESE, ARTURO;VITALE, MONICA;CAPASSO, Mario;MARONE, GIANNI;ZAMBRANO, NICOLA
2011
Abstract
Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. Using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7 allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
