Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. Using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7 allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy
SPADARO, GIUSEPPE;GENOVESE, ARTURO;VITALE, MONICA;CAPASSO, Mario;MARONE, GIANNI;ZAMBRANO, NICOLA
2011
Abstract
Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. Using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7 allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
