Alkaline incubation of NADH results in the formation of a very potent inhibitor of lactate dehydrogenase. High resolution mass spectroscopy along with NMR characterization clearly showed that the inhibitor is derived from attachment of a glycolic acid moiety to the 4-position of the dihydronicotinamide ring of NADH. The very potent inhibitor is competitive with respect to NADH. The inhibitor added in submicromolar concentrations to cardiomyocytes protects them from damage caused by hypoxia/reoxygenation stress. In isolated mouse hearts, addition of the inhibitor results in a substantial reduction of myocardial infarct size caused by global ischemia/reperfusion injury.
Cardioprotective Activity of A Novel and Potent Competitive Inhibitor of Lactate Dehydrogenase / A. B., Kotlyar; Randazzo, Antonio; N., Honbo; Z. Q., Jin; J. S., Karliner; G., Cecchini. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 584:(2010), pp. 159-165. [10.1016/j.febslet.2009.11.022]
Cardioprotective Activity of A Novel and Potent Competitive Inhibitor of Lactate Dehydrogenase
RANDAZZO, ANTONIO;
2010
Abstract
Alkaline incubation of NADH results in the formation of a very potent inhibitor of lactate dehydrogenase. High resolution mass spectroscopy along with NMR characterization clearly showed that the inhibitor is derived from attachment of a glycolic acid moiety to the 4-position of the dihydronicotinamide ring of NADH. The very potent inhibitor is competitive with respect to NADH. The inhibitor added in submicromolar concentrations to cardiomyocytes protects them from damage caused by hypoxia/reoxygenation stress. In isolated mouse hearts, addition of the inhibitor results in a substantial reduction of myocardial infarct size caused by global ischemia/reperfusion injury.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
