The mixed effects of nitrogen nutrition and sulphate assimilation were investigated in barley plants (Hordeum vulgare var. Alfeo) that were subjected to long-term sulphur and/or nitrogen starvation, by measuring the O-acetylserine(thio)lyase (OASTL-EC 4.2.99.8) activity, changes in -SH compounds and amino acid levels. The growth of barley plants cultured in the hydroponic vessels was severely affected by altered nutrient levels. The barley plants grown in medium deprived of nitrogen and/or sulphur sources for 21 days showed increase in both root length and weight. In contrast, the shoot growth was reduced in nitrogen-starved plants and was unaffected by sulphur deprivation. Sulphur starvation affected the level of proteins in barley plants more than nitrogen deprivation. The decline in the protein levels observed under sulphur-deficient conditions was coupled with the accumulation of glutamine, asparagine and serine, mainly in the roots; additionally, a nitrogen deficiency in the roots promoted a decrease in both glutathione and cysteine levels.

Nitrogen assimilation and cysteine biosynthesis in barley: Evidence for rootsulphur assimilation upon recovery from N deprivation / Carfagna, Simona; Vona, Vincenza; DI MARTINO, Vittoria; Esposito, Sergio; Rigano, C.. - In: ENVIRONMENTAL AND EXPERIMENTAL BOTANY. - ISSN 0098-8472. - 71:(2011), pp. 18-24. [10.1016/j.envexpbot.2010.10.008]

Nitrogen assimilation and cysteine biosynthesis in barley: Evidence for rootsulphur assimilation upon recovery from N deprivation

CARFAGNA, SIMONA;VONA, VINCENZA;DI MARTINO, VITTORIA;ESPOSITO, SERGIO;
2011

Abstract

The mixed effects of nitrogen nutrition and sulphate assimilation were investigated in barley plants (Hordeum vulgare var. Alfeo) that were subjected to long-term sulphur and/or nitrogen starvation, by measuring the O-acetylserine(thio)lyase (OASTL-EC 4.2.99.8) activity, changes in -SH compounds and amino acid levels. The growth of barley plants cultured in the hydroponic vessels was severely affected by altered nutrient levels. The barley plants grown in medium deprived of nitrogen and/or sulphur sources for 21 days showed increase in both root length and weight. In contrast, the shoot growth was reduced in nitrogen-starved plants and was unaffected by sulphur deprivation. Sulphur starvation affected the level of proteins in barley plants more than nitrogen deprivation. The decline in the protein levels observed under sulphur-deficient conditions was coupled with the accumulation of glutamine, asparagine and serine, mainly in the roots; additionally, a nitrogen deficiency in the roots promoted a decrease in both glutathione and cysteine levels.
2011
Nitrogen assimilation and cysteine biosynthesis in barley: Evidence for rootsulphur assimilation upon recovery from N deprivation / Carfagna, Simona; Vona, Vincenza; DI MARTINO, Vittoria; Esposito, Sergio; Rigano, C.. - In: ENVIRONMENTAL AND EXPERIMENTAL BOTANY. - ISSN 0098-8472. - 71:(2011), pp. 18-24. [10.1016/j.envexpbot.2010.10.008]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/372234
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