Stearoyl-CoA desaturase (SCD) plays a key metabolic role by changing the saturated FA content of ruminant milk and meat. In this study we characterized for the first time the stearoyl-CoA desaturase (SCD) gene in river buffalo (Bubalus bubalis) and investigated its genetic variability. On a total of 78 buffaloes, 15 SNPs were detected and 6 of them were preliminarily genotyped. In particular, the g.133A>C SNP was found to create a new consensus site for the SP1 binding site, thus generating a new tandem repeat in the promoter region. A preliminary association study with the milk fatty acid content showed that the C allele significantly affects the total desaturation index (P < 0.01). Linkage disequilibrium analysis allowed identification of 7 haplotypes and 4 tag SNPs. Such polymorphisms could represent useful genetic markers for association studies with fatty acid composition, but further studies are needed to evaluate their potential use to improve the nutritional quality of the dairy products.

Sequence analysis and genetic variability of stearoyl CoA desaturase (SCD) gene in the Italian Mediterranean river buffalo / Pauciullo, Alfredo; Cosenza, Gianfranco; D’Avino, A.; Colimoro, L.; Nicodemo, Davide; Coletta, A.; Feligini, M.; Marchitelli, C.; DI BERARDINO, Dino; Ramunno, Luigi. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - STAMPA. - 24:6(2010), pp. 407-410. [10.1016/j.mcp.2010.07.009]

Sequence analysis and genetic variability of stearoyl CoA desaturase (SCD) gene in the Italian Mediterranean river buffalo

PAUCIULLO, ALFREDO;COSENZA, GIANFRANCO;NICODEMO, DAVIDE;DI BERARDINO, DINO;RAMUNNO, LUIGI
2010

Abstract

Stearoyl-CoA desaturase (SCD) plays a key metabolic role by changing the saturated FA content of ruminant milk and meat. In this study we characterized for the first time the stearoyl-CoA desaturase (SCD) gene in river buffalo (Bubalus bubalis) and investigated its genetic variability. On a total of 78 buffaloes, 15 SNPs were detected and 6 of them were preliminarily genotyped. In particular, the g.133A>C SNP was found to create a new consensus site for the SP1 binding site, thus generating a new tandem repeat in the promoter region. A preliminary association study with the milk fatty acid content showed that the C allele significantly affects the total desaturation index (P < 0.01). Linkage disequilibrium analysis allowed identification of 7 haplotypes and 4 tag SNPs. Such polymorphisms could represent useful genetic markers for association studies with fatty acid composition, but further studies are needed to evaluate their potential use to improve the nutritional quality of the dairy products.
2010
Sequence analysis and genetic variability of stearoyl CoA desaturase (SCD) gene in the Italian Mediterranean river buffalo / Pauciullo, Alfredo; Cosenza, Gianfranco; D’Avino, A.; Colimoro, L.; Nicodemo, Davide; Coletta, A.; Feligini, M.; Marchitelli, C.; DI BERARDINO, Dino; Ramunno, Luigi. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - STAMPA. - 24:6(2010), pp. 407-410. [10.1016/j.mcp.2010.07.009]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/371530
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 16
social impact