An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%.
Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry / Avitabile, C.; Moggio, L.; D’Andrea, L. D.; Pedone, C.; Romanelli, Alessandra. - In: TETRAHEDRON LETTERS. - ISSN 0040-4039. - STAMPA. - 51:29(2010), pp. 3716-3718. [10.1016/j.tetlet.2010.05.026]
Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry
ROMANELLI, ALESSANDRA
2010
Abstract
An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
