Transferrin receptor (CD71), a membrane glycoprotein involved in iron absorption, that plays a key role in metabolism and cell homeostasis, is a biomarker of X-ray damage in man and rat(1). Following previous observations by FACS and immunocytochemical detection at L.M., that suggested an increased CD71 expression in the circulating erythroid cells (RBCs) of Torpedoes after X-ray exposure, the aim of this study was the ultrastructural localization of this marker before and after X-ray treatment of benthonic Elasmobranchs (Torpedo marmorata and Torpedo ocellata) in order to evaluate whether CD71 can be used as a biomarker of sea X-ray pollution. Ultrathin sections of RBCs, withdrawn from 3 specimens before and after irradiation (45, 80 and 90 Gy), were first incubated with polyclonal primary antibody anti-CD71, then with secondary antibody conjugated with colloidal gold and lastly contrasted and observed by T.E.M.. Before irradiation, only few RBCs showed low CD71-immunoreactivity, mainly in plasma membrane but also cytoplasm. After irradiation, morphological apoptotic-like changes in the nucleus and cytoplasm vacuolization were observed and a more intense CD71-ir was found mainly into cytoplasmic vesicles, demonstrating an increased receptor endocytosis and occasionally in mitochondria, injured by irradiation. These preliminary data, showing an increased CD71 expression and internalization in circulating RBCs of Torpedoes after X-ray exposure, may suggest, if confirmed by further experiments, the role of CD71 as biomarker of X-ray sea pollution.
Ultrastructural immunolocalization of transferrin receptor in circulating erythroid cells: its role as biomarker for X ray damage in Elasmobranchs / Basile, Filomena; Occhiello, Antonella; C. A., Glomski; Pica, Alessandra. - STAMPA. - volume 53/ supplement 1:(2009), pp. 37-37. (Intervento presentato al convegno 33rd National Congress of the Italian Society of Histochemistry tenutosi a Rome nel 8-10 June 2009).
Ultrastructural immunolocalization of transferrin receptor in circulating erythroid cells: its role as biomarker for X ray damage in Elasmobranchs
BASILE, FILOMENA;OCCHIELLO, ANTONELLA;PICA, ALESSANDRA
2009
Abstract
Transferrin receptor (CD71), a membrane glycoprotein involved in iron absorption, that plays a key role in metabolism and cell homeostasis, is a biomarker of X-ray damage in man and rat(1). Following previous observations by FACS and immunocytochemical detection at L.M., that suggested an increased CD71 expression in the circulating erythroid cells (RBCs) of Torpedoes after X-ray exposure, the aim of this study was the ultrastructural localization of this marker before and after X-ray treatment of benthonic Elasmobranchs (Torpedo marmorata and Torpedo ocellata) in order to evaluate whether CD71 can be used as a biomarker of sea X-ray pollution. Ultrathin sections of RBCs, withdrawn from 3 specimens before and after irradiation (45, 80 and 90 Gy), were first incubated with polyclonal primary antibody anti-CD71, then with secondary antibody conjugated with colloidal gold and lastly contrasted and observed by T.E.M.. Before irradiation, only few RBCs showed low CD71-immunoreactivity, mainly in plasma membrane but also cytoplasm. After irradiation, morphological apoptotic-like changes in the nucleus and cytoplasm vacuolization were observed and a more intense CD71-ir was found mainly into cytoplasmic vesicles, demonstrating an increased receptor endocytosis and occasionally in mitochondria, injured by irradiation. These preliminary data, showing an increased CD71 expression and internalization in circulating RBCs of Torpedoes after X-ray exposure, may suggest, if confirmed by further experiments, the role of CD71 as biomarker of X-ray sea pollution.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
