In this work, we have quantified the functionalization of some porous silicon multilayers that optically act as Bragg reflectors: 1.3 x 10(-3) mol/g, corresponding to 3.25 nmol/cm(2), of a ten base oligonucleotide has been estimated by a chemical procedure, based on the dimethoxytrityl monitoring. The oligonucleotide has been synthesized directly on two different porous silicon structures, one oxidized by a thermal treatment, the other by a chemical process based on exposure to I-2/pyridine. We have also monitored the oligonucleotide synthesis by a label-free technique: a 11 nm red-shift of the optical spectrum can be observed by spectroscopic reflectometry.

Direct Synthesis of Oligonucleotides on Nanostructured Silica Multilayers

REA, ILARIA;OLIVIERO, GIORGIA;AMATO, JUSSARA;BORBONE, NICOLA;PICCIALLI, GENNARO;DE STEFANO, Luca
2010

Abstract

In this work, we have quantified the functionalization of some porous silicon multilayers that optically act as Bragg reflectors: 1.3 x 10(-3) mol/g, corresponding to 3.25 nmol/cm(2), of a ten base oligonucleotide has been estimated by a chemical procedure, based on the dimethoxytrityl monitoring. The oligonucleotide has been synthesized directly on two different porous silicon structures, one oxidized by a thermal treatment, the other by a chemical process based on exposure to I-2/pyridine. We have also monitored the oligonucleotide synthesis by a label-free technique: a 11 nm red-shift of the optical spectrum can be observed by spectroscopic reflectometry.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/366490
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